Nedospasov S A, Shakhov A N, Sali R
Mol Biol (Mosk). 1984 Jul-Aug;18(4):1090-8.
Structure of SV40 wild type virus genome differing from the well known 776 strain has been characterized. This particular strain (SV40) was used previously for viral chromatin analysis. We show here that SV40 strain differs from 776 strain by deletion in the beginning of the "late" region (enhancer). Restriction nucleases mapping and nucleotide sequencing through this region reveal the absence of one full-length copy of 72 bp repeat.
与著名的776株不同的SV40野生型病毒基因组结构已得到表征。这种特殊的毒株(SV40)先前用于病毒染色质分析。我们在此表明,SV40毒株与776毒株的不同之处在于“晚期”区域(增强子)起始处的缺失。通过该区域的限制性核酸酶图谱分析和核苷酸测序揭示了72bp重复序列的一个全长拷贝缺失。