Fossel E T
Department of Radiology, Beth Israel Hospital and Harvard Medical School, Boston, Massachusetts 02215.
Cancer Cells. 1991 May;3(5):173-82.
Our laboratory has developed nuclear magnetic resonance (NMR) techniques for detecting cancer. Using water-suppressed proton (H-1) NMR spectroscopy, we observed that the linewidths of the resonances of methyl and methylene moieties in lipoprotein lipids were consistently narrower in plasma samples from cancer patients than in those from controls. These findings have been corroborated by a number of independent laboratories, but other investigators have been unable to reproduce our results. One reason for the variability of results obtained with H-1 NMR may be that hypertriglyceridemia also induces linewidth narrowing of lipoprotein lipid methyl and methylene resonances, and can cause false positive results. We show that this ambiguity can be circumvented by using a second test based on the carbon-13 (C-13) NMR spectrum of plasma. Here we postulate that the cancer-associated changes seen in H-1 and C-13 NMR spectra are caused by peroxidation of lipoprotein lipids, an effect that may be induced by tumor necrosis factor-alpha released during malignancy.
我们实验室已开发出用于检测癌症的核磁共振（NMR）技术。使用水抑制质子（H-1）核磁共振光谱法，我们观察到癌症患者血浆样本中脂蛋白脂质的甲基和亚甲基部分共振峰的线宽始终比对照组样本中的窄。许多独立实验室都证实了这些发现，但其他研究人员未能重现我们的结果。使用H-1 NMR获得的结果存在差异的一个原因可能是高甘油三酯血症也会导致脂蛋白脂质甲基和亚甲基共振峰的线宽变窄，并可能导致假阳性结果。我们表明，通过基于血浆碳-13（C-13）核磁共振光谱的第二项测试可以避免这种模糊性。在此我们假定，在H-1和C-13 NMR光谱中看到的与癌症相关的变化是由脂蛋白脂质的过氧化作用引起的，这种作用可能是由恶性肿瘤期间释放的肿瘤坏死因子-α诱导的。
