Levy N S, Bakalyar H A, Reed R R
Howard Hughes Medical Institute Laboratory of Genetics, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
J Steroid Biochem Mol Biol. 1991 Oct;39(4B):633-7. doi: 10.1016/0960-0760(91)90262-4.
Recent efforts in our laboratory have focused on cloning the molecular components involved in the cAMP-mediated pathway of olfactory signal transduction. These efforts have resulted in the isolation of olfactory-specific forms of a G protein, an adenylyl cyclase, and a cyclic nucleotide-gated cation channel. Functional expression of each of these proteins in vitro confirms their ability to carry out the function ascribed to them as part of a second-messenger cascade. Putative odorant-receptor molecules which constitute the first step in odorant signal transduction have now been cloned. We have generated oligonucleotide probes which recognize a population of olfactory receptors apparently more heterogeneous than those previously reported. These probes should enable us to answer questions regarding the number of different receptors expressed per cell as well as the nature of receptor-ligand specificity.
我们实验室最近的工作重点是克隆嗅觉信号转导的cAMP介导途径中涉及的分子成分。这些工作已导致分离出一种G蛋白、一种腺苷酸环化酶和一种环核苷酸门控阳离子通道的嗅觉特异性形式。这些蛋白质在体外的功能表达证实了它们作为第二信使级联反应的一部分执行赋予它们的功能的能力。构成气味信号转导第一步的假定气味受体分子现已被克隆。我们已经生成了寡核苷酸探针，这些探针识别的一群嗅觉受体明显比以前报道的更具异质性。这些探针将使我们能够回答关于每个细胞表达的不同受体数量以及受体-配体特异性性质的问题。
