一种用于快速诊断传染性胃肠炎病毒的捕获酶免疫测定法。

A capture-enzyme immunoassay for rapid diagnosis of transmissible gastroenteritis virus.

作者信息

Lu W, Osorio F A, Rhodes M B, Moxley R A

机构信息

Department of Veterinary Science, University of Nebraska-Lincoln 68583.

出版信息

J Vet Diagn Invest. 1991 Apr;3(2):119-23. doi: 10.1177/104063879100300202.

DOI:10.1177/104063879100300202

PMID:1654131

Abstract

Two enzyme immunoassays (EIA) were developed for the detection of swine transmissible gastroenteritis virus (TGEV) antigens. The 2 EIAs used the same detecting system, a monoclonal antibody conjugated to horseradish peroxidase, but used different capture systems including a monoclonal antibody (m-EIA) or a polyclonal antibody (p-EIA). The EIAs were compared with the fluorescent antibody test (FAT) and electron microscopy (EM) for the detection of TGEV in intestinal samples of experimentally inoculated gnotobiotic piglets and of conventional diarrheic pigs submitted for diagnosis. In the gnotobiotic piglets experimentally inoculated with TGEV, 81.8% (9/11) were positive for TGEV by p-EIA, and 72.7% (8/11) were positive by m-EIA. In comparison, 81.8% (9/11) were positive by FAT and 27.2% (3/11) were positive by EM. Three noninfected controls were negative by all tests. In the diagnostic samples, 86.0% (43/50) were positive by p-EIA, 68.2% (30/44) were positive by m-EIA, 28.6% (14/49) were positive by IFA, and 38.0% (19/50) were positive by EM. The m-EIA had a higher agreement with FAT and EM than did p-EIA.

摘要

开发了两种酶免疫测定法（EIA）用于检测猪传染性胃肠炎病毒（TGEV）抗原。这两种EIA使用相同的检测系统，即与辣根过氧化物酶偶联的单克隆抗体，但使用不同的捕获系统，包括单克隆抗体（m-EIA）或多克隆抗体（p-EIA）。将这些EIA与荧光抗体试验（FAT）和电子显微镜检查（EM）进行比较，以检测实验接种的无菌仔猪和提交诊断的常规腹泻猪的肠道样本中的TGEV。在实验接种TGEV的无菌仔猪中，p-EIA检测TGEV的阳性率为81.8%（9/11），m-EIA的阳性率为72.7%（8/11）。相比之下，FAT的阳性率为81.8%（9/11），EM的阳性率为27.2%（3/11）。三个未感染的对照在所有检测中均为阴性。在诊断样本中，p-EIA的阳性率为86.0%（43/50），m-EIA的阳性率为68.2%（30/44），IFA的阳性率为28.6%（14/49），EM的阳性率为38.0%（19/50）。m-EIA与FAT和EM的一致性高于p-EIA。