基于单克隆抗体的夹心酶联免疫吸附测定法与病毒分离法在山羊组织和分泌物中检测小反刍兽疫病毒的比较
Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.
作者信息
Saliki J T, House J A, Mebus C A, Dubovi E J
机构信息
Foreign Animal Disease Diagnostic Laboratory, U.S. Department of Agriculture, Plum Island, Greenport, New York 11944.
出版信息
J Clin Microbiol. 1994 May;32(5):1349-53. doi: 10.1128/jcm.32.5.1349-1353.1994.
Abstract
A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.
摘要
开发了一种基于单克隆抗体的夹心酶联免疫吸附测定法（S-ELISA），用于特异性检测小反刍兽疫病毒。使用来自六只实验感染山羊的89对组织和分泌物样本，与在Vero细胞培养物中进行病毒分离相比，S-ELISA的灵敏度显著更高（71.9%对65.2%；P<0.05）。S-ELISA是病毒分离的合适替代方法。
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