人类2型副流感病毒与猿猴病毒41型和5型之间的分子关系：猿猴病毒41型和5型核蛋白基因序列的测定

Molecular relationships between human parainfluenza virus type 2, and simian viruses 41 and 5: determination of nucleoprotein gene sequences of simian viruses 41 and 5.

作者信息

Tsurudome M, Oki N, Higuchi Y, Miyahara K, Yoshimoto M, Mutsuga N, Kitada S, Ogawa M, Miyahara Y, Okamoto K

机构信息

Department of Microbiology, Mie University School of Medicine, Japan.

出版信息

J Gen Virol. 1991 Sep;72 ( Pt 9):2289-92. doi: 10.1099/0022-1317-72-9-2289.

DOI:10.1099/0022-1317-72-9-2289

PMID:1654380

Abstract

The nucleotide sequences of cDNAs of the simian virus 5 (SV5) nucleoprotein (NP) gene, and the 3' end of the genome and NP gene of SV41 were determined. The open reading frames of the SV5 and SV41 NP genes encode polypeptides with Mrs of 56,582 and 60,575, respectively, values which are consistent with those estimated by SDS-PAGE. The NP of human parainfluenza virus type 2 (hPIV-2) was more closely related to that of SV41 (amino acid sequence identity 70.5%) than that of SV5 (57.0%); the amino acid sequence identity between the NPs of SV41 and SV5 was 63.3%. The sequence of the 3' end of the genome of SV41 showed a high level of similarity to that of hPIV-2, the terminal 18 nucleotides being identical. It is concluded from these findings that SV41 is related most closely to hPIV-2, even though SV5 had been thought to be an animal type of hPIV-2.

摘要

测定了猿猴病毒5（SV5）核蛋白（NP）基因的cDNA核苷酸序列，以及SV41基因组和NP基因的3'端序列。SV5和SV41 NP基因的开放阅读框分别编码分子量为56,582和60,575的多肽，这些值与通过SDS-PAGE估计的值一致。人副流感病毒2型（hPIV-2）的NP与SV41的NP关系更为密切（氨基酸序列同一性为70.5%），而与SV5的NP关系相对较远（57.0%）；SV41和SV5的NP之间的氨基酸序列同一性为63.3%。SV41基因组3'端的序列与hPIV-2的序列具有高度相似性，末端18个核苷酸完全相同。从这些发现可以得出结论，尽管SV5曾被认为是hPIV-2的动物型，但SV41与hPIV-2的关系最为密切。