Zhou Zeng-Hui, Liu Li, Xiao Fan-Rong, Wang Gui-Ying, Xu Zhi-Zhan
State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Dec;25(12):1986-90.
R6G was incubated in three kinds of silver colloid with different low concentrations, and its surface-enhanced resonance Raman scattering (SERRS) was studied with a confocal microscopy system. Each sample exhibited different spectrum character. At the single-molecule level the SERRS-spectra were recorded in 10(-13) mol x L(-1) dye colloidal solution. Some spectral inhomogeneous behavior was recorded such as spectral diffusion, intensity fluctuation of vibrational lines and spectral polarization, and even on/off blinking and splitting of some lines within the spectrum of one molecule. Polarization of excitation light and background subtraction of signal have an important influence on the SERRS spectra detection of single-molecule, which was analyzed in the present paper.
将R6G与三种不同低浓度的银胶体进行孵育，并用共聚焦显微镜系统研究其表面增强共振拉曼散射（SERRS）。每个样品都表现出不同的光谱特征。在单分子水平上，在10^(-13) mol·L^(-1)染料胶体溶液中记录了SERRS光谱。记录到了一些光谱不均匀行为，如光谱扩散、振动线强度波动和光谱偏振，甚至在一个分子的光谱内某些谱线的开/关闪烁和分裂。激发光的偏振和信号的背景扣除对单分子SERRS光谱检测有重要影响，本文对此进行了分析。
