Habuchi Satoshi, Cotlet Mircea, Gronheid Roel, Dirix Gunter, Michiels Jan, Vanderleyden Jos, De Schryver Frans C, Hofkens Johan
Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium.
J Am Chem Soc. 2003 Jul 16;125(28):8446-7. doi: 10.1021/ja0353311.
In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level.
在本论文中，我们证明了可获得来自单个绿色荧光蛋白（GFP）的表面增强共振拉曼散射光谱。最重要的发现是通过相应的振动指纹图谱在单分子水平上直接检测发色团去质子化和质子化形式之间的转换，以及增强型绿色荧光蛋白（EGFP）也显示出高表面增强共振拉曼散射（SERRS）信号这一事实。我们的研究结果表明了该技术在单分子水平上研究蛋白质分子结构动力学的潜力。
