The purified 14-kilodalton envelope protein of vaccinia virus produced in Escherichia coli induces virus immunity in animals.
作者信息
Lai C F, Gong S C, Esteban M
机构信息
Department of Biochemistry, State University of New York, Brooklyn 11203.
出版信息
J Virol. 1991 Oct;65(10):5631-5. doi: 10.1128/JVI.65.10.5631-5635.1991.
Abstract
Vaccinia virus (VV) was successfully used as a live vaccine to eradicate smallpox, but the nature of viral proteins involved in eliciting viral immunity has not yet been identified. A potential candidate is a 14-kDa VV envelope protein that is involved in virus penetration at the level of virus-cell fusion, in cell-cell fusion late in infection, and in virus dissemination. The 14-kDa envelope protein has been produced in Escherichia coli, with properties similar to those of the native protein found in the virus particle and in infected cells (C. Lai, S. Gong, and M. Esteban, J. Biol. Chem. 256:22174-22180, 1990). In this investigation, we showed that mice immunized with purified VV 14-kDa protein synthesized in E. coli in the form of a monomer or a trimer develop high-titer neutralizing antibodies and are protected when challenged with lethal doses of wild-type VV. Our findings demonstrate that it is possible to confer protection against VV through immunization with the 14-kDa envelope protein.
摘要
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本文引用的文献
1
Significance of extracellular enveloped virus in the in vitro and in vivo dissemination of vaccinia.细胞外被膜病毒在痘苗病毒体外和体内传播中的意义。
J Gen Virol. 1980 Sep;50(1):89-100. doi: 10.1099/0022-1317-50-1-89.
2
The smallpox story: life and death of an old disease.天花的故事：一种古老疾病的生与死
Microbiol Rev. 1983 Dec;47(4):455-509. doi: 10.1128/mr.47.4.455-509.1983.
3
Isolation and characterization of neutralizing monoclonal antibodies to vaccinia virus.痘苗病毒中和单克隆抗体的分离与鉴定
J Virol. 1985 Nov;56(2):482-8. doi: 10.1128/JVI.56.2.482-488.1985.
4
A 14,000-Mr envelope protein of vaccinia virus is involved in cell fusion and forms covalently linked trimers.痘苗病毒的一种14000道尔顿的包膜蛋白参与细胞融合并形成共价连接的三聚体。
J Virol. 1987 Feb;61(2):395-404. doi: 10.1128/JVI.61.2.395-404.1987.
5
Isolation and characterization of attenuated mutants of vaccinia virus.牛痘病毒减毒突变体的分离与鉴定
Virology. 1987 Aug;159(2):408-22. doi: 10.1016/0042-6822(87)90480-6.
6
Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals.萤火虫荧光素酶基因在痘苗病毒中的表达：一种用于追踪病毒在感染动物组织中传播的高度敏感的基因标记物。
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71. doi: 10.1073/pnas.85.5.1667.
7
Vaccinia: virus, vector, vaccine.牛痘病毒：病毒、载体、疫苗。
Adv Virus Res. 1988;34:43-64. doi: 10.1016/s0065-3527(08)60515-1.
8
Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.噬斑大小表型作为一种选择标记来产生痘苗病毒重组体。
J Virol. 1989 Feb;63(2):997-1001. doi: 10.1128/JVI.63.2.997-1001.1989.
9
Mapping and nucleotide sequence of the vaccinia virus gene that encodes a 14-kilodalton fusion protein.编码一种14千道尔顿融合蛋白的痘苗病毒基因的图谱及核苷酸序列。
J Virol. 1987 Nov;61(11):3550-4. doi: 10.1128/JVI.61.11.3550-3554.1987.
10
A single point mutation of Ala-25 to Asp in the 14,000-Mr envelope protein of vaccinia virus induces a size change that leads to the small plaque size phenotype of the virus.痘苗病毒14000道尔顿包膜蛋白中Ala - 25突变为Asp的单点突变会导致大小变化，从而产生该病毒的小蚀斑大小表型。
J Virol. 1989 Nov;63(11):4507-14. doi: 10.1128/JVI.63.11.4507-4514.1989.
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