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噬斑大小表型作为一种选择标记来产生痘苗病毒重组体。

Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants.

作者信息

Rodriguez J F, Esteban M

机构信息

Department of Biochemistry, Health Science Center, State University of New York, Brooklyn 11203.

出版信息

J Virol. 1989 Feb;63(2):997-1001. doi: 10.1128/JVI.63.2.997-1001.1989.

DOI:10.1128/JVI.63.2.997-1001.1989
PMID:2911129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247783/
Abstract

In this report, we provide a new method for selection of vaccinia virus recombinants expressing foreign genes. The method is based on the use of the gene encoding the viral 14,000-molecular-weight envelope protein that rescues the small-plaque-size phenotype of a vaccinia virus variant to large-plaque-size virus. Selection of recombinants is easily obtained after visual inspection of large viral plaques.

摘要

在本报告中，我们提供了一种选择表达外源基因的痘苗病毒重组体的新方法。该方法基于使用编码病毒14,000分子量包膜蛋白的基因，该基因可将痘苗病毒变体的小噬斑大小表型挽救为大噬斑大小的病毒。通过目视检查大型病毒噬斑后，很容易获得重组体的选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84d2/247783/b5315a14dd35/jvirol00069-0542-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84d2/247783/d4696ab26944/jvirol00069-0541-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84d2/247783/b5315a14dd35/jvirol00069-0542-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84d2/247783/d4696ab26944/jvirol00069-0541-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84d2/247783/b5315a14dd35/jvirol00069-0542-a.jpg

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本文引用的文献

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Vaccinia virus: a selectable eukaryotic cloning and expression vector.痘苗病毒：一种可选择的真核克隆和表达载体。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9. doi: 10.1073/pnas.79.23.7415.
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Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.痘病毒作为克隆载体的构建：将单纯疱疹病毒的胸苷激酶基因插入有感染性的痘苗病毒DNA中。
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Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype.
CRISPR/Cas9-推动正痘病毒基因组编辑用于疫苗和载体开发。
Viruses. 2018 Jan 22;10(1):50. doi: 10.3390/v10010050.
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Methodology for the efficient generation of fluorescently tagged vaccinia virus proteins.高效生成荧光标记痘苗病毒蛋白的方法
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Recombinant vaccinia viruses. Design, generation, and isolation.重组痘苗病毒。设计、产生及分离
Mol Biotechnol. 1999 Dec 15;13(3):223-45. doi: 10.1385/MB:13:3:223.
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Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety.用于重组基因表达、疫苗接种和安全性的基因工程痘病毒。
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11341-8. doi: 10.1073/pnas.93.21.11341.
7
A single point mutation of Ala-25 to Asp in the 14,000-Mr envelope protein of vaccinia virus induces a size change that leads to the small plaque size phenotype of the virus.痘苗病毒14000道尔顿包膜蛋白中Ala - 25突变为Asp的单点突变会导致大小变化，从而产生该病毒的小蚀斑大小表型。
J Virol. 1989 Nov;63(11):4507-14. doi: 10.1128/JVI.63.11.4507-4514.1989.
8
Vaccinia virus vectors: new strategies for producing recombinant vaccines.痘苗病毒载体：生产重组疫苗的新策略。
Clin Microbiol Rev. 1990 Apr;3(2):153-70. doi: 10.1128/CMR.3.2.153.
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6
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J Virol. 1987 Feb;61(2):395-404. doi: 10.1128/JVI.61.2.395-404.1987.
7
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J Virol. 1987 Aug;61(8):2642-7. doi: 10.1128/JVI.61.8.2642-2647.1987.
9
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Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71. doi: 10.1073/pnas.85.5.1667.
10
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Mol Cell Biol. 1985 Aug;5(8):1918-24. doi: 10.1128/mcb.5.8.1918-1924.1985.