Akiyoshi T T, Kimura N, Uike N, Kozuru M, Tamura K, Takihara Y, Hisano S, Nishimura J, Kikuchi M
First Department of Internal Medicine, Fukuoka University School of Medicine, Japan.
Leuk Res. 1991;15(8):683-91. doi: 10.1016/0145-2126(91)90070-a.
We have analysed the configuration of immunoassociated genes and the karyotypes of 30 patients with acute myelocytic leukemia (AML) and 10 with chronic myelocytic leukemia in blast crisis (CML-BC). In AML, the frequencies of T-cell receptor (TcR) beta, gamma, and delta chain and immunoglobulin heavy and light chain gene rearrangements were 4.2%, 19%, 8%, 10.7% and 10.5%, respectively. In CML-BC, they were 10%, 20%, 40%, 50% and 0%, respectively. Nine patients had abnormalities in chromosome 2, 7 or 14, upon which immunoassociated genes are located. There seems to be no apparent relationship between these chromosome abnormalities and gene rearrangements. In all patients but one (5/6), the delta rearrangement was accompanied by other immunoassociated gene rearrangements. Molecular size analysis revealed specific delta rearranged band(s) (19.5 kb-BamHI and/or 6.9 kb-EcoRI), as commonly detected in B-acute lymphocytic leukemia (ALL). All the patients with the delta rearranged band, however, had a germline configuration of J delta gene loci, suggesting a DD or V(D)D (probably V delta 2(D)D) pattern. This study also indicates that the delta rearrangement is specific in AML or CML-BC and distinct from that in early T leukemia/lymphoma.
我们分析了30例急性髓细胞白血病（AML）患者和10例慢性髓细胞白血病急变期（CML-BC）患者的免疫相关基因构型和核型。在AML中，T细胞受体（TcR）β、γ和δ链以及免疫球蛋白重链和轻链基因重排的频率分别为4.2%、19%、8%、10.7%和10.5%。在CML-BC中，它们分别为10%、20%、40%、50%和0%。9例患者在2号、7号或14号染色体上存在异常，而免疫相关基因位于这些染色体上。这些染色体异常与基因重排之间似乎没有明显关系。在除1例患者外的所有患者中（5/6），δ重排伴有其他免疫相关基因重排。分子大小分析显示出特定的δ重排条带（19.5 kb-BamHI和/或6.9 kb-EcoRI），这在B急性淋巴细胞白血病（ALL）中常见。然而，所有具有δ重排条带的患者Jδ基因座均为种系构型，提示为DD或V(D)D（可能是Vδ2(D)D）模式。本研究还表明，δ重排在AML或CML-BC中具有特异性，且与早期T白血病/淋巴瘤中的不同。
