Ikeda T, Kita K, Miwa H, Kawakami K, Anazawa H, Ohno T, Nosaka T, Hatanaka M, Honjo T, Shirakawa S
Second Department of International Medicine, Faculty of Medicine, Mie University, Tsu, Japan.
Leuk Res. 1990;14(2):195-201. doi: 10.1016/0145-2126(90)90049-f.
Fourteen patients with lymphoid and mixed blast crisis (BC) of chronic myelocytic leukemia were studied by immunophenotyping and genotyping. Rearrangements of immunoglobulin heavy chain (IgH), T-cell receptor (TcR) gamma and TcR beta genes were detected in all 14, in nine and in four patients, respectively. Interestingly, more than two rearranged bands of IgH gene in three lymphoid BC and two rearranged bands with germ line band in 1 biphenotypic BC indicated the genetic heterogeneity of the blasts. Some blastic transformations are thought to occur at a more immature stage of hematopoietic differentiation than that indicated by the phenotype and genotype of BC cells.
通过免疫表型分析和基因分型对14例慢性粒细胞白血病淋巴样和混合原始细胞危象（BC）患者进行了研究。在所有14例患者中均检测到免疫球蛋白重链（IgH）、T细胞受体（TcR）γ和TcRβ基因重排，分别有9例和4例患者检测到上述基因重排。有趣的是，3例淋巴样BC患者中IgH基因有两条以上重排条带，1例双表型BC患者中有两条重排条带和一条种系条带，这表明原始细胞存在基因异质性。一些原始细胞转化被认为发生在造血分化比BC细胞表型和基因型所显示的更不成熟的阶段。
