Kikawa Y, Hosoi S, Inoue Y, Saito M, Nakai A, Shigematsu Y, Hirao T, Sudo M
Department of Pediatrics, Fukui Medical School, Japan.
Pediatr Res. 1991 May;29(5):455-9. doi: 10.1203/00006450-199105010-00009.
Urinary levels of leukotriene (LT) E4, a stable end-product of LTC4 and LTD4, were measured before and after exercise in 10 children with severe asthma and seven children with moderate asthma using HPLC and RIA to clarify the relationship of LT to the severity of asthma and to the degree of bronchospasm in exercise-induced asthma. The urinary LTE4 level significantly increased after exercise in the severe asthma group, but not in the moderate asthma group (14.3 +/- 14.5 to 24.3 +/- 20.6 versus 19.6 +/- 12.3 to 17.6 +/- 10.8 ng/mmol creatinine, p less than 0.05). The urinary LTE4 level increased in 10 patients (eight with severe asthma), and it decreased in seven patients (five with moderate asthma). A significant difference in the degree of bronchospasm after exercise (as shown by the maximal % fall in the peak expiratory flow rate), was seen when patients with increased urinary LTE4 excretion were compared with those with decreased excretion (60.4 +/- 17.3 versus 24.1 +/- 14.3%, p less than 0.01). Our findings suggest that exercise-induced asthma, or at least a subtype of exercise-induced asthma, may partly develop through the release of LTC4.
采用高效液相色谱法（HPLC）和放射免疫分析法（RIA），对10名重度哮喘患儿及7名中度哮喘患儿运动前后白三烯（LT）E4（LTC4和LTD4的稳定终产物）的尿水平进行测定，以阐明LT与哮喘严重程度以及运动诱发性哮喘中支气管痉挛程度之间的关系。重度哮喘组运动后尿LTE4水平显著升高，而中度哮喘组则未升高（分别为14.3±14.5至24.3±20.6与19.6±12.3至17.6±10.8 ng/mmol肌酐，p<0.05）。10例患者（8例重度哮喘）尿LTE4水平升高，7例患者（5例中度哮喘）尿LTE4水平降低。将尿LTE4排泄增加的患者与排泄减少的患者相比，运动后支气管痉挛程度存在显著差异（最大呼气流量峰值下降百分比分别为60.4±17.3与24.1±14.3%，p<0.01）。我们的研究结果表明，运动诱发性哮喘，或至少运动诱发性哮喘的一种亚型，可能部分通过LTC4的释放而发生。
