Sabolová D, Kozurková M, Kristian P, Danihel I, Podhradský D, Imrich J
Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P.J. Safárik University, 041 54 Kosice, Slovakia.
Int J Biol Macromol. 2006 Mar 30;38(2):94-8. doi: 10.1016/j.ijbiomac.2006.01.015. Epub 2006 Mar 20.
Novel acridine derivatives, wherein the steric factor has been varied systematically through substitution at the 9 position of the acridine ring, were evaluated as convenient fluorescent probes for nucleic acid detection. The binding affinities of N-(9-acridinylthiocarbamoyl)amino acids (ATA) with plasmid DNA (pUC 19) were investigated using UV-vis spectrophotometry, fluorometric titration and quantum chemical calculations (AM1). From spectrofluorometric analysis, the binding constants for the DNA-ATA complexes were determined. To elucidate its DNA intercalation, the most preferable tautomeric structure of ATA was established by means of AM1 calculations.
新型吖啶衍生物，其中通过在吖啶环的9位进行取代，空间因素已被系统地改变，被评估为用于核酸检测的便捷荧光探针。使用紫外可见分光光度法、荧光滴定法和量子化学计算（AM1）研究了N-（9-吖啶基硫代甲酰基）氨基酸（ATA）与质粒DNA（pUC 19）的结合亲和力。通过荧光光谱分析，确定了DNA-ATA复合物的结合常数。为了阐明其对DNA的嵌入作用，通过AM1计算确定了ATA最优选的互变异构结构。
