Allan Ian J, Semple Kirk T, Hare Rina, Reid Brian J
School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
Environ Pollut. 2006 Nov;144(2):562-71. doi: 10.1016/j.envpol.2006.01.026. Epub 2006 Mar 20.
In this study, an aqueous-based hydroxypropyl-beta-cyclodextrin (HPCD) extraction technique was assessed for its capacity to determine the microbially degradable fraction of mono- and polycyclic aromatic hydrocarbons in four dissimilar soils. A linear relationship (slope=0.90; R2=0.89), approaching 1:1 between predicted and observed phenanthrene mineralization, was demonstrated for the cyclodextrin extraction; however, the water only extraction underestimated the microbially available fraction by a factor of three (slope=3.35; R2=0.64). With respect to determining the mineralizable fraction of p-cresol in soils, the cyclodextrin extraction (slope=0.94; R2=0.84) was more appropriate than the water extraction (slope=1.50; R2=0.36). Collectively, these results suggested that the cyclodextrin extraction technique was suitable for the prediction of the mineralizable fraction of representative PAHs and phenols present in dissimilar soils following increasing soil-contaminant contact times. The assessment of the microbial availability of contaminants in soils is important for a more representative evaluation of soil contamination.
在本研究中，评估了基于水的羟丙基-β-环糊精（HPCD）提取技术测定四种不同土壤中单环和多环芳烃微生物可降解部分的能力。环糊精提取显示出预测的菲矿化与观察到的菲矿化之间接近1:1的线性关系（斜率 = 0.90；R2 = 0.89）；然而，仅用水提取会将微生物可利用部分低估三倍（斜率 = 3.35；R2 = 0.64）。关于测定土壤中对甲酚的可矿化部分，环糊精提取（斜率 = 0.94；R2 = 0.84）比水提取（斜率 = 1.50；R2 = 0.36）更合适。总体而言，这些结果表明，随着土壤与污染物接触时间增加，环糊精提取技术适用于预测不同土壤中代表性多环芳烃和酚类的可矿化部分。评估土壤中污染物的微生物可利用性对于更具代表性地评估土壤污染很重要。
