Yamamoto Kimiko, Narukawa Junko, Kadono-Okuda Keiko, Nohata Junko, Sasanuma Motoe, Suetsugu Yoshitaka, Banno Yutaka, Fujii Hiroshi, Goldsmith Marian R, Mita Kazuei
Genome Research Department, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
Genetics. 2006 May;173(1):151-61. doi: 10.1534/genetics.105.053801. Epub 2006 Mar 17.
We have developed a linkage map for the silkworm Bombyx mori based on single nucleotide polymorphisms (SNPs) between strains p50T and C108T initially found on regions corresponding to the end sequences of bacterial artificial chromosome (BAC) clones. Using 190 segregants from a backcross of a p50T female x an F1 (p50T x C108T) male, we analyzed segregation patterns of 534 SNPs between p50T and C108T, detected among 3840 PCR amplicons, each associated with a p50T BAC end sequence. This enabled us to construct a linkage map composed of 534 SNP markers spanning 1305 cM in total length distributed over the expected 28 linkage groups. Of the 534 BACs whose ends harbored the SNPs used to construct the linkage map, 89 were associated with 107 different ESTs. Since each of the SNP markers is directly linked to a specific genomic BAC clone and to whole-genome sequence data, and some of them are also linked to EST data, the SNP linkage map will be a powerful tool for investigating silkworm genome properties, mutation mapping, and map-based cloning of genes of industrial and agricultural interest.
我们基于最初在与细菌人工染色体（BAC）克隆末端序列相对应区域发现的p50T和C108T品系之间的单核苷酸多态性（SNP），构建了家蚕的连锁图谱。利用p50T雌蚕与F1（p50T×C108T）雄蚕回交产生的190个分离后代，我们分析了在3840个PCR扩增产物中检测到的p50T和C108T之间534个SNP的分离模式，每个扩增产物都与一个p50T BAC末端序列相关。这使我们能够构建一个由534个SNP标记组成的连锁图谱，总长度为1305厘摩，分布在预期的28个连锁群上。在用于构建连锁图谱的534个末端含有SNP的BAC中，89个与107个不同的EST相关。由于每个SNP标记都直接与特定的基因组BAC克隆和全基因组序列数据相连，其中一些还与EST数据相连，因此SNP连锁图谱将成为研究家蚕基因组特性、突变定位以及工农业相关基因图位克隆的有力工具。
