Valladares S, Sánchez C, Martínez M T, Ballester A, Vieitez A M
Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avda. de Vigo s/n, Apartado 122, 15080, Santiago de Compostela, Spain.
Plant Cell Rep. 2006 Sep;25(9):879-86. doi: 10.1007/s00299-005-0108-z. Epub 2006 Mar 18.
Somatic embryogenesis was induced in expanding leaf explants excised from epicormic shoots forced from branch segments taken at four different times of year from a mature oak (Quercus robur L.). Branch segments 2-4 cm in diameter produced most shoots when collected in March. Somatic embryos were induced on explants derived from branches of all collection dates, although collection in November seemed to afford the best results. Germination and conversion ability of embryos of embryogenic lines derived from six oak trees depended heavily on genotype, conversion rates ranging from 0 to 70%. RAPD analyses found no evidence of genetic variation either within or between the embryogenic lines established from three of these trees, or between these lines and the trees of origin, or between somatic embryo derived plantlets and the trees of origin. The embryogenic system used in this study appears to be suitable for true-to-type clonal propagation of mature oak genotypes.
从一棵成年栎树（欧洲栓皮栎）一年中四个不同时间采集的枝条段上促发的隐芽所切取的正在伸展的叶片外植体中诱导出了体细胞胚胎发生。直径2 - 4厘米的枝条段在3月份采集时产生的芽最多。所有采集日期的枝条所获得的外植体上都能诱导出体细胞胚胎，不过11月份采集的似乎效果最佳。来自六棵栎树的胚性系的胚胎的萌发和转化能力在很大程度上取决于基因型，转化率在0%至70%之间。随机扩增多态性DNA分析没有发现从其中三棵树建立的胚性系内部或之间、这些胚性系与原始树木之间、体细胞胚胎衍生的植株与原始树木之间存在遗传变异的证据。本研究中使用的胚性系统似乎适用于成年栎树基因型的真实类型克隆繁殖。
