过氧化氢处理的哺乳动物细胞中体内DNA碱基损伤的化学性质。

Chemical nature of in vivo DNA base damage in hydrogen peroxide-treated mammalian cells.

作者信息

Dizdaroglu M, Nackerdien Z, Chao B C, Gajewski E, Rao G

机构信息

Center for Chemical Technology, National Institute of Standards and Technology, Gaithersburg, Maryland 20899.

出版信息

Arch Biochem Biophys. 1991 Mar;285(2):388-90. doi: 10.1016/0003-9861(91)90378-v.

DOI:10.1016/0003-9861(91)90378-v

PMID:1654775

Abstract

Hydrogen peroxide is generated in mammalian cells by normal metabolism or by treatment with external agents. Treatment of mammalian cells with this oxidizing agent results in DNA damage. Little is known about the chemical nature of hydrogen peroxide-mediated DNA damage in mammalian cells. Here we report on the chemical characterization of in vivo base damage to nuclear DNA in mammalian cells caused by exposure to H2O2. Chromatin was isolated from cells and analyzed by gas chromatography/mass spectrometry with selected-ion monitoring. Ten DNA base products were identified and quantitated. Modified bases identified were typical hydroxyl radical-induced products of DNA bases. Results indicate involvement of hydroxyl radicals in the mechanism of nuclear DNA damage in mammalian cells caused by H2O2.

摘要

过氧化氢在哺乳动物细胞中可通过正常代谢或经外部试剂处理产生。用这种氧化剂处理哺乳动物细胞会导致DNA损伤。关于过氧化氢介导的哺乳动物细胞DNA损伤的化学性质，人们了解甚少。在此，我们报告了暴露于H2O2后哺乳动物细胞中核DNA体内碱基损伤的化学特征。从细胞中分离出染色质，并通过气相色谱/质谱联用的选择离子监测进行分析。鉴定并定量了10种DNA碱基产物。鉴定出的修饰碱基是典型的DNA碱基羟基自由基诱导产物。结果表明羟基自由基参与了H2O2导致的哺乳动物细胞核DNA损伤机制。