血清和密度受限的成纤维细胞培养物中环状AMP和环状GMP的浓度
Cyclic AMP and cyclic GMP concentrations in serum- and density-restricted fibroblast cultures.
作者信息
Moens W, Vokaer A, Kram R
出版信息
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1063-7. doi: 10.1073/pnas.72.3.1063.
Abstract
Mouse fibroblasts transformed by simian virus 40 (SV3T3 cells) are characterized by cyclic AMP and cyclic GMP levels, respectively, about half and twice those found in growing untransformed 3T3 cells. Density-dependent inhibition of growth is correlated with reduced cyclic GMP concentrations in 3T3 and four different density-restricted revertant lines derived from SV3T3. The levels of cyclic AMP are not increased at confluence. Upon serum restriction, serum-dependent cell lines show a greater increase in intracellular cAMP than serum-insensitive lines. Cyclic GMP levels are greatly reduced, even in serum-insensitive density revertants, but not in SV3T3. Serum readdition to all serum-dependent lines is followed by a rapid decrease in cyclic AMP and increase in cyclic GMP concentrations. The magnitude of these responses is decreased in SV3T3 and density revertants.
摘要
由猴病毒40转化的小鼠成纤维细胞（SV3T3细胞）的特征在于，其环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平分别约为未转化的生长中的3T3细胞的一半和两倍。生长的密度依赖性抑制与3T3细胞以及源自SV3T3的四个不同密度限制回复系中cGMP浓度的降低相关。汇合时cAMP水平不会升高。血清限制后，血清依赖性细胞系的细胞内cAMP升高幅度大于血清不敏感细胞系。即使在血清不敏感的密度回复系中，cGMP水平也会大幅降低，但在SV3T3细胞中不会。所有血清依赖性细胞系重新添加血清后，cAMP会迅速降低，cGMP浓度会升高。在SV3T3细胞和密度回复系中，这些反应的幅度会降低。
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