Su Shu-Hui, Chen Hsiun-ing, Jen Chauying J
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Chin J Physiol. 2005 Dec 31;48(4):210-6.
Severe exercise augments the phagocytic capability of bronchoalveolar macrophages (BAMs) in the absence of pulmonary surfactant, a lung immunity modulator in vivo. This study was to investigate whether the exercise effect on BAM phagocytosis is partially mediated by surfactant components. Male BALB/c mice (9-12 wk old) were divided into control and severe exercise groups. Mice in the exercise group received progressive treadmill running exercise until exhaustion. BAMs and lung lavage supernatant were collected under either sedentary or post-severe exercise conditions. Phagocytosis of IgG/C'-opsonized beads by BAMs was determined in the presence of lavage supernatant. Mannose, a monosaccharide competitor for the carbohydrate recognition domain of surfactant protein A (SP-A), and SP-A antibodies were applied to examine the role of SP-A in the exercise-induced facilitating effects on BAM phagocytosis. BAMs from either control or post-exercise animals had elevated phagocytosis of IgG/C'-opsonized beads when incubated with autologous lung lavage supernatant. The supernatant-mediated increase in BAM phagocytosis of IgG/C'-opsonized beads was dose-dependently inhibited by mannose or SP-A antibodies. In addition, higher concentrations of SP-A inhibitors were needed to inhibit BAM phagocytosis in post-exercise group than that in the control group. We also observed that SP-A inhibitors were ineffective in the absence of lung lavage supernatant. Furthermore, post-exercise, but not control, BAMs displayed time-dependent alterations in their membrane-bound SP-A amount during 30-min incubation with autologous lung lavage supernatant. SP-A plays a major role in the severe exercise-enhanced surfactant-mediated BAM phagocytosis.
在缺乏肺表面活性物质（一种体内肺免疫调节剂）的情况下，剧烈运动可增强支气管肺泡巨噬细胞（BAM）的吞噬能力。本研究旨在调查运动对BAM吞噬作用的影响是否部分由表面活性物质成分介导。将雄性BALB/c小鼠（9 - 12周龄）分为对照组和剧烈运动组。运动组小鼠进行渐进式跑步机跑步运动直至 exhaustion。在久坐或剧烈运动后条件下收集BAM和肺灌洗上清液。在存在灌洗上清液的情况下测定BAM对IgG/C'-调理珠的吞噬作用。甘露糖（一种表面活性蛋白A（SP-A）碳水化合物识别域的单糖竞争者）和SP-A抗体用于研究SP-A在运动诱导的促进BAM吞噬作用中的作用。当与自体肺灌洗上清液一起孵育时，来自对照组或运动后动物的BAM对IgG/C'-调理珠的吞噬作用增强。甘露糖或SP-A抗体剂量依赖性地抑制上清液介导的BAM对IgG/C'-调理珠吞噬作用的增加。此外，与对照组相比，运动后组需要更高浓度的SP-A抑制剂来抑制BAM吞噬作用。我们还观察到在没有肺灌洗上清液时SP-A抑制剂无效。此外，运动后而非对照组的BAM在与自体肺灌洗上清液孵育30分钟期间，其膜结合SP-A量呈现时间依赖性变化。SP-A在剧烈运动增强的表面活性物质介导的BAM吞噬作用中起主要作用。
