Su S H, Chen H I, Jen C J
Department of Physiology, College of Medicine, National Cheng-Kung University, Tainan, Taiwan, Republic of China.
J Leukoc Biol. 2001 Jan;69(1):75-80.
Because physical activity affects the immune competency of individuals by an unknown mechanism, we investigated the effect of acute exercise on phagocytosis of bronchoalveolar macrophages (BAMs). Male BALB/c mice, 7-9 weeks old, ran on a treadmill to exhaustion (severe exercise, SE) or at a final speed of 17 m/min for 30 min (moderate exercise, ME). Although both exercise protocols induced differential leukocytosis, 95% leukocytes from lung lavages of both groups were BAMs. The BAM phagocytic capacity of nonopsonized beads increased immediately after SE but not after ME, gradually returning to the basal level after 4 h. SE upregulates the macrophage scavenger receptors (SR-A type I/II and MARCO), CR3, and ICAM-1, but not Fc gammaR. Although the blocking effect of MARCO antibody was most pronounced, that of ICAM-1 antibody was totally reversed by cross-linking CR3. Our results showed that SE, but not ME, activated BAMs and that the enhanced nonopsonized phagocytosis was mainly mediated by scavenger receptors and ICAM-1/CR3.
由于体育活动通过未知机制影响个体的免疫能力，我们研究了急性运动对支气管肺泡巨噬细胞（BAM）吞噬作用的影响。7至9周龄的雄性BALB/c小鼠在跑步机上跑步直至精疲力竭（剧烈运动，SE）或以17米/分钟的最终速度跑30分钟（适度运动，ME）。尽管两种运动方案均诱导了不同程度的白细胞增多，但两组肺灌洗中的白细胞有95%是BAM。非调理素化珠子的BAM吞噬能力在SE后立即增加，但在ME后未增加，4小时后逐渐恢复到基础水平。SE上调巨噬细胞清道夫受体（I/II型SR-A和MARCO）、CR3和ICAM-1，但不上调FcγR。尽管MARCO抗体的阻断作用最为明显，但ICAM-1抗体的阻断作用通过交联CR3被完全逆转。我们的结果表明，SE而非ME激活了BAM，增强的非调理素化吞噬作用主要由清道夫受体和ICAM-1/CR3介导。
