Bloch Konstantin, Papismedov Eli, Yavriyants Karina, Vorobeychik Marina, Beer Sven, Vardi Pnina
Diabetes and Obesity Research Laboratory, Felsenstein Medical Research Center, Sackler Faculty of Medicine, Tel-Aviv University, Beilinson Campus, Petah Tikva, Israel.
Tissue Eng. 2006 Feb;12(2):337-44. doi: 10.1089/ten.2006.12.337.
Immunoisolation of pancreatic islets interrupts their vascular connections and results in severe cell hypoxia and dysfunction. This process is believed to be the major obstacle to a successful cure of diabetes by implantation of bioartificial pancreas. Here we describe a new technology for microalga-based, photosynthetic oxygen supply to encapsulated islets, in which a thermophylic strain of the unicellular alga Chlorella was used as a natural photosynthetic oxygen generator. Following determinations of the optimal number of alga cells required for compensation of islet respiration, an appropriate number of islets and algae were co-encapsulated in alginate and perifused with oxygen-free medium at increasing glucose concentrations. No insulin response to glucose was obtained in islets alone, or upon inactivation of photosynthesis by darkness. However, under illumination, photosynthetic- dependent oxygen generation induced higher glucose-stimulated insulin response when compared to normoxic perifusion. Such photosynthetic oxygen generation may have a potential application in development of various bioartificial tissues, in particular the endocrine pancreas.
胰岛的免疫隔离会中断其血管连接，导致严重的细胞缺氧和功能障碍。这一过程被认为是通过植入生物人工胰腺成功治愈糖尿病的主要障碍。在此，我们描述了一种基于微藻的新技术，用于为封装的胰岛提供光合氧气供应，其中单细胞藻类小球藻的嗜热菌株被用作天然光合氧气发生器。在确定补偿胰岛呼吸所需的最佳藻细胞数量后，将适当数量的胰岛和藻类共同封装在藻酸盐中，并在不断增加的葡萄糖浓度下用无氧培养基进行灌流。单独的胰岛或在黑暗中使光合作用失活时，未观察到对葡萄糖的胰岛素反应。然而，在光照条件下，与常氧灌流相比，光合依赖的氧气生成诱导了更高的葡萄糖刺激胰岛素反应。这种光合氧气生成可能在各种生物人工组织的开发中具有潜在应用，特别是内分泌胰腺。
