Pratt R M, Martin G R
Proc Natl Acad Sci U S A. 1975 Mar;72(3):874-7. doi: 10.1073/pnas.72.3.874.
Cyclic AMP levels increase abruptly in the secondary palatal shelf (mesenchyme plus a two- to three-cell-layered epithelium) between day 15 and 16 of gestation in the rat embryo. The addition of dibutyryl cyclic AMP to the media in which immature palatal shelves (day 14) are cultured causes a precocious decrease in DNA synthesis, but increased glycoprotein synthesis and epithelial adhesiveness. These changes are most evident in the medial-edge epithelial cells and resemble the developmental events that occur normally on day 15 and 16 during formation of the secondary palate. The initiation of epithelial cell death and adhesiveness in the medial edge of the palatal shelf may be mediated through an increase in cyclic AMP.
在大鼠胚胎妊娠第15至16天期间，次生腭架（间充质加上两到三层细胞的上皮）中的环磷酸腺苷（cAMP）水平会突然升高。向培养未成熟腭架（第14天）的培养基中添加二丁酰环磷酸腺苷会导致DNA合成过早减少，但糖蛋白合成增加以及上皮黏附性增强。这些变化在边缘内侧上皮细胞中最为明显，并且类似于次生腭形成过程中第15和16天正常发生的发育事件。腭架边缘内侧上皮细胞死亡和黏附性的启动可能是通过环磷酸腺苷的增加介导的。
