Basile D V, Wood H N, Braun A C
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3055-9. doi: 10.1073/pnas.70.11.3055.
An attempt was made in this study to determine more precisely the nature of the factors that are involved in the programming of cells for a form of terminal cellular differentiation that results in death. These studies demonstrated that both the cytokinesins, which are potent inhibitors of plant and animal adenosine 3':5'-cyclic monophosphate phosphodiesterases, and 8-bromoadenosine 3':5'-cyclic monophosphate, which is a stable, biologically active form of adenosine 3':5'-cyclic monophosphate, are highly effective in encouraging differentiation of parenchyma cells into tracheary elements with accompanying death. Since adenosine 3':5'-cyclic monophosphate and theophylline when used together were also effective, the results reported here suggest that adenosine 3':5'-cyclic monophosphate is somehow importantly involved in the conversion of parenchyma cells into tracheary elements in this system. The possible significance to the tumor problem generally of the programming of cells for terminal differentiation, with or without resulting death, is discussed.
本研究试图更精确地确定参与细胞编程以实现一种导致死亡的终末细胞分化形式的因素的性质。这些研究表明，细胞分裂素（动植物腺苷3':5'-环磷酸二酯酶的有效抑制剂）和8-溴腺苷3':5'-环磷酸（腺苷3':5'-环磷酸的一种稳定的生物活性形式）在促使薄壁细胞分化为管状分子并伴随细胞死亡方面都非常有效。由于腺苷3':5'-环磷酸和茶碱一起使用时也有效，因此本文报道的结果表明，腺苷3':5'-环磷酸在该系统中以某种重要方式参与了薄壁细胞向管状分子的转化。本文还讨论了细胞编程以进行终末分化（无论是否导致死亡）对肿瘤问题的一般潜在意义。
