Sokol F, Koprowski H
Proc Natl Acad Sci U S A. 1975 Mar;72(3):933-6. doi: 10.1073/pnas.72.3.933.
Recently accumulated knowledge allows more precise comparison of the structural (and possibly evolutionary) relationships of several different animal rhabdoviruses: vesicular stomatitis virus, rabies virus, Kern Canyon virus, and spring viremia of carp virus. Each virus is composed primarily of a glycoprotein, an RNA-associated nucleoprotein, and one or two membrane proteins. Vesicular stomatitis virus group viruses contain lesser amounts of two additional distinct polypeptides, NS and L. The separate viruses undergo structural polypeptide phosphorylation in vivo according to characteristic patterns. In vesicular stomatitis virus the NS protein is selectively phosphorylated. In rabies group viruses and in spring viremia of carp virus, the nucleoprotein is the predominant phosphoprotein; in these viruses only the phosphorylated moiety is selectively cleaved off with trypsin. In Kern Canyon virus, only membrane protein and glycoprotein are weakly phosphorylated. Each virus possesses a virion-bound protein kinase. Vesicular stomatitis virus group viruses, Kern Canyon virus, and spring viremia of carp virus only contain virion-bound transcriptases of respectively decreasing levels of activity demonstrable in vitro. Vesicular stomatitis and Kern Canyon viruses replicate efficiently in enucleated cells; rabies virus does not. Based upon these observations, it is suggested that vesicular stomatitis virus may represent the most highly evolved of these rhabdoviruses, whereas spring viremia of carp and Kern Canyon viruses may represent "evolutionary links" between the vesicular stomatitis and rabies virus groups.
最近积累的知识使人们能够更精确地比较几种不同动物弹状病毒的结构（以及可能的进化）关系：水疱性口炎病毒、狂犬病病毒、克恩峡谷病毒和鲤鱼春季病毒血症病毒。每种病毒主要由一种糖蛋白、一种与RNA相关的核蛋白以及一种或两种膜蛋白组成。水疱性口炎病毒群病毒还含有少量另外两种不同的多肽，即NS和L。这些不同的病毒在体内会根据特定模式进行结构多肽磷酸化。在水疱性口炎病毒中，NS蛋白被选择性磷酸化。在狂犬病病毒群病毒和鲤鱼春季病毒血症病毒中，核蛋白是主要的磷蛋白；在这些病毒中，只有磷酸化部分能被胰蛋白酶选择性切割。在克恩峡谷病毒中，只有膜蛋白和糖蛋白被微弱磷酸化。每种病毒都拥有一种与病毒粒子结合的蛋白激酶。水疱性口炎病毒群病毒、克恩峡谷病毒和鲤鱼春季病毒血症病毒只含有与病毒粒子结合的转录酶，其体外可检测到的活性水平依次降低。水疱性口炎病毒和克恩峡谷病毒能在去核细胞中高效复制；狂犬病病毒则不能。基于这些观察结果，有人提出水疱性口炎病毒可能代表了这些弹状病毒中进化程度最高的，而鲤鱼春季病毒血症病毒和克恩峡谷病毒可能代表了水疱性口炎病毒群和狂犬病病毒群之间的“进化联系”。
