Geist L J, Monick M M, Stinski M F, Hunninghake G W
Department of Medicine, Department of Veterans Affairs Medical Center, Iowa City, Iowa.
Am J Respir Cell Mol Biol. 1991 Sep;5(3):292-6. doi: 10.1165/ajrcmb/5.3.292.
Human cytomegalovirus (HCMV) immediate early (IE) genes act as trans-acting factors to upregulate various viral promoters. We used various IE plasmid constructs in transient transfection assays and demonstrated that the HCMV IE2 gene product upregulated expression from the interleukin (IL)-2 and IL-2 receptor (IL-2R) promoters and increased amounts of endogenous, steady-state IL-2 and IL-2R RNA. In marked contrast, the IE1 gene product, which can upregulate the major IE promoter and the IL-1 beta promoter, had no effect on the IL-2 and IL-2R promoters. These studies suggest a role for the HCMV IE2 gene product as a modulator of the inflammatory response associated with HCMV infection.
人巨细胞病毒（HCMV）立即早期（IE）基因作为反式作用因子上调各种病毒启动子。我们在瞬时转染试验中使用了各种IE质粒构建体，并证明HCMV IE2基因产物上调了白细胞介素（IL）-2和IL-2受体（IL-2R）启动子的表达，并增加了内源性稳态IL-2和IL-2R RNA的量。与之形成显著对比的是，能够上调主要IE启动子和IL-1β启动子的IE1基因产物对IL-2和IL-2R启动子没有影响。这些研究表明HCMV IE2基因产物作为与HCMV感染相关的炎症反应调节剂发挥作用。
