Murthy Ganti S, Singh Vijay, Johnston David B, Rausch Kent D, Tumbleson M E
Agricultural and Biological Engineering Department, University of Illinois at Urbana-Champaign, 360G, AESB, 1304 West Pennsylvania Avenue, Urbana, IL 61801, USA.
J Ind Microbiol Biotechnol. 2006 Aug;33(8):655-60. doi: 10.1007/s10295-006-0109-0. Epub 2006 Mar 21.
With rapid growth of fuel ethanol industry, and concomitant increase in distillers dried grains with solubles (DDGS), new corn fractionation technologies that reduce DDGS volume and produce higher value coproducts in dry grind ethanol process have been developed. One of the technologies, a dry degerm, defiber (3D) process (similar to conventional corn dry milling) was used to separate germ and pericarp fiber prior to the endosperm fraction fermentation. Recovery of germ and pericarp fiber in the 3D process results in removal of lipids from the fermentation medium. Biosynthesis of lipids, which is important for cell growth and viability, cannot proceed in strictly anaerobic fermentations. The effects of ten different lipid supplements on improving fermentation rates and ethanol yields were studied and compared to the conventional dry grind process. Endosperm fraction (from the 3D process) was mixed with water and liquefied by enzymatic hydrolysis and was fermented using simultaneous saccharification and fermentation. The highest ethanol concentration (13.7% v/v) was achieved with conventional dry grind process. Control treatment (endosperm fraction from 3D process without lipid supplementation) produced the lowest ethanol concentration (11.2% v/v). Three lipid treatments (fatty acid ester, alkylphenol, and ethoxylated sorbitan ester 1836) were most effective in improving final ethanol concentrations. Fatty acid ester treatment produced the highest final ethanol concentration (12.3% v/v) among all lipid supplementation treatments. Mean final ethanol concentrations of alkylphenol and ethoxylated sorbitan ester 1836 supplemented samples were 12.3 and 12.0% v/v, respectively.
随着燃料乙醇产业的快速发展以及干酒糟及其可溶物(DDGS)产量的相应增加,人们开发了新的玉米分馏技术,以减少DDGS的产量,并在干法磨碎乙醇工艺中生产出更具价值的副产品。其中一项技术是干脱胚、脱纤维(3D)工艺(类似于传统的玉米干法碾磨),用于在胚乳分馏发酵之前分离胚芽和果皮纤维。3D工艺中胚芽和果皮纤维的回收可去除发酵培养基中的脂质。脂质的生物合成对细胞生长和活力很重要,在严格的厌氧发酵中无法进行。研究了十种不同脂质补充剂对提高发酵速率和乙醇产量的影响,并与传统干法磨碎工艺进行了比较。(来自3D工艺的)胚乳部分与水混合,通过酶水解液化,然后采用同步糖化发酵法进行发酵。传统干法磨碎工艺获得了最高乙醇浓度(13.7% v/v)。对照处理(来自3D工艺的胚乳部分,未添加脂质)产生了最低乙醇浓度(11.2% v/v)。三种脂质处理(脂肪酸酯、烷基酚和聚氧乙烯山梨糖醇酐单油酸酯1836)在提高最终乙醇浓度方面最有效。在所有脂质补充处理中,脂肪酸酯处理产生了最高的最终乙醇浓度(12.3% v/v)。添加烷基酚和聚氧乙烯山梨糖醇酐单油酸酯1836的样品的平均最终乙醇浓度分别为12.3% v/v和12.0% v/v。