Otto S, Baum T, Keller F
Institute of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig.
Ann Anat. 2006 Mar;188(2):153-8. doi: 10.1016/j.aanat.2005.08.017.
The aim of the current study was to find out whether there are sex-dependent differences in the relative number of elastic fibres in human heart valves. Twenty-six aortic valves, 26 mitral valves, 33 pulmonary valves and 28 tricuspid valves of both sexes were obtained at autopsy from newborn to 89-year-old patients who died of noncardiac diseases. The quantitatively morphometric investigations were carried out on conventionally stained (Resorcinfuchsin) histological sections. The results were qualitatively examined with immuno-histochemically marked (anti-elastin antibodies) histological sections. Earlier examinations by Leutert [1976. Z. Gesamte Inn. Med. 31, 97-104] showed that the atrioventricular valves have the following layers: endothelium, atrial fibroelastic tissue (S1), fibrous tissue, ventricular fibroelastic tissue (S2) and endothelium. In our study, the ventricular side of the semilunar valves corresponds to side S1, whereas the vessel side corresponds to side S2. Three regions of interest were examined on each side of the valves: base, mid and tip. The number of elastic fibres per measuring area for all four human heart valves was significantly higher (p < 0,001) in fibroelastic tissue of side S1 than in fibroelastic tissue of side S2. Neither on side S1 nor on side S2 were there significant gender-related differences in the relative number of elastic fibres per measuring area. The results suggest a characteristic distribution of the elastic fibre system which is not sex-dependent but closely related to the function of the heart valves.
本研究的目的是查明人类心脏瓣膜中弹性纤维的相对数量是否存在性别差异。从死于非心脏疾病的新生儿至89岁患者的尸检中获取了26个主动脉瓣、26个二尖瓣、33个肺动脉瓣和28个三尖瓣，涵盖了男女两性。对常规染色（间苯二酚品红）的组织学切片进行了定量形态学研究。并用免疫组织化学标记（抗弹性蛋白抗体）的组织学切片对结果进行了定性检查。Leutert[1976. Z. Gesamte Inn. Med. 31, 97 - 104]早期的研究表明，房室瓣有以下几层：内皮、心房纤维弹性组织（S1）、纤维组织、心室纤维弹性组织（S2）和内皮。在我们的研究中，半月瓣的心室侧对应于S1侧，而血管侧对应于S2侧。在瓣膜的每一侧检查了三个感兴趣的区域：基部、中部和尖端。所有四种人类心脏瓣膜在S1侧的纤维弹性组织中，每测量面积的弹性纤维数量显著高于（p < 0.001）S2侧的纤维弹性组织。在S1侧和S2侧，每测量面积弹性纤维的相对数量均无显著的性别相关差异。结果表明弹性纤维系统具有特征性分布，它不依赖于性别，而是与心脏瓣膜的功能密切相关。
