Keller F, Werner R, Wähner J, Köhler T, Wolff W, Leutert G
Institut für Anatomie Universitätsklinikum Leipzig.
Z Gerontol Geriatr. 1999 Apr;32(2):104-11. doi: 10.1007/s003910050091.
Previous polarization-microscopical measurements combined with silver-staining methods at histological sections of the bicuspid valve and the tricuspid valve in the region of fibrosa showed (similar to that of the corresponding tendinous cords) for middle-aged persons a distinct difference in the collagen composition in comparison between high pressure vs. low pressure system. The aim of the current study was the morphometric investigation of the collagen composition, of the relative content of total collagen (measured as relative number of total collagen fibers per measuring area), and of the cell-collagen relation in dependence of age and possibly on sex in the high and low pressure system, respectively. Tissue samples of atrioventricular valves of 67 probands of both sexes were available. The probands were healthy with regard to heart and circulation and distributed in 3 age groups (1st to 2nd decade, 3rd to 5th decade, and 6th to 9th decade). After corresponding preliminary treatment, the histological sections of tissue samples were evaluated by a suitable combination of polarization-microscopical, immuno-histochemical, and morphometrical methodology. The thickness of the heart valves was measured by microscopic image analysis. A rise of the number of fibers per measuring area (ma) for both sexes and both valves was observed with increasing age. The slopes of the linear regression curves and the mean values of the numbers of collagenous fibers/ma (for each age group) were different between both sexes. They depend upon the valve considered. Analogous to these results, the differentiated statements were possible to the number of fibers/ma and to the percentages of the fiber species by the distinction between the collagen types I and III in the fibrosa of the heart valves. Whereas the relative numbers of fibers/ma rose with increasing age, the number of cell (fibroblasts, fibrocytes) nuclei for both valves and sexes slightly decreased. For the latter, there was no difference between high and low pressure system in contrast to the collagenous fibers. The opposite age-dependent behavior of the collagenous fibers and of their producing cells during the biomorphosis of the heart valves could be explained with the collagen turn-over.
先前在二尖瓣和三尖瓣纤维区域的组织切片上，通过偏振显微镜测量并结合银染法显示（类似于相应的腱索），对于中年人群，高压系统与低压系统相比，胶原成分存在明显差异。本研究的目的是分别对高压系统和低压系统中胶原成分、总胶原相对含量（以每测量区域总胶原纤维的相对数量衡量）以及细胞 - 胶原关系随年龄以及可能随性别的变化进行形态计量学研究。有67名男女受试者的房室瓣组织样本。这些受试者心脏和循环系统健康，分布在3个年龄组（10至20岁、30至50岁、60至90岁）。经过相应的预处理后，通过偏振显微镜、免疫组织化学和形态计量学方法的适当组合对组织样本的组织切片进行评估。通过显微镜图像分析测量心脏瓣膜的厚度。随着年龄增长，观察到男女两性以及两个瓣膜每测量区域的纤维数量均增加。两性的线性回归曲线斜率以及（每个年龄组的）胶原纤维数量/每测量区域的平均值不同。它们取决于所考虑的瓣膜。与这些结果类似，通过区分心脏瓣膜纤维层中I型和III型胶原，可以对纤维数量/每测量区域以及纤维种类的百分比做出不同的表述。虽然纤维数量/每测量区域的相对数量随年龄增长而增加，但两个瓣膜和两性的细胞（成纤维细胞、纤维细胞）核数量略有减少。对于后者，与胶原纤维不同，高压系统和低压系统之间没有差异。心脏瓣膜生物形态形成过程中胶原纤维及其产生细胞的相反年龄依赖性行为可以用胶原周转来解释。
