N-甲基-D-天冬氨酸（NMDA）受体拮抗剂MK-801可阻断非阿片类应激诱导的镇痛作用。I. 阿片受体缺陷型和阿片受体丰富型小鼠品系的比较。

N-methyl-D-aspartic acid (NMDA) receptor antagonist MK-801 blocks non-opioid stress-induced analgesia. I. Comparison of opiate receptor-deficient and opiate receptor-rich strains of mice.

作者信息

Marek P, Page G G, Ben-Eliyahu S, Liebeskind J C

机构信息

Department of Psychology, University of California, Los Angeles 90024.

出版信息

Brain Res. 1991 Jun 14;551(1-2):293-6. doi: 10.1016/0006-8993(91)90943-p.

DOI:10.1016/0006-8993(91)90943-p

PMID:1655162

Abstract

The effects of the specific N-methyl-D-aspartic acid (NMDA) receptor antagonist MK-801 (0.075 mg/kg), and the specific opiate receptor antagonist naloxone (10 mg/kg), on swim stress-induced analgesia (SSIA) were studied in opiate receptor-deficient (CXBK) and opiate receptor-rich (CXBH) mice. Animals were subjected to forced swimming, and analgesia was assessed using the hot-plate test. In CXBK mice SSIA was blocked by MK-801 but was completely insensitive to naloxone. In CXBH mice SSIA was partially attenuated both by naloxone and MK-801, and it was nearly abolished by a combination of these drugs. Morphine analgesia (10 mg/kg) was abolished by naloxone but completely unaffected by MK-801 in CXBH mice. These findings suggest that the NMDA receptor is critically involved in the non-opioid component of SSIA.

摘要

在缺乏阿片受体的（CXBK）小鼠和富含阿片受体的（CXBH）小鼠中，研究了特异性N-甲基-D-天冬氨酸（NMDA）受体拮抗剂MK-801（0.075毫克/千克）和特异性阿片受体拮抗剂纳洛酮（10毫克/千克）对游泳应激诱导镇痛（SSIA）的影响。对动物进行强迫游泳，并使用热板试验评估镇痛效果。在CXBK小鼠中，SSIA被MK-801阻断，但对纳洛酮完全不敏感。在CXBH小鼠中，SSIA被纳洛酮和MK-801部分减弱，并且这两种药物联合使用时几乎完全消除。在CXBH小鼠中，纳洛酮可消除吗啡镇痛（10毫克/千克），但MK-801对其完全无影响。这些发现表明，NMDA受体在SSIA的非阿片类成分中起关键作用。