Razzaghi-Abyaneh Mehdi, Shams-Ghahfarokhi Masoomeh, Eslamifar Ali, Schmidt Oskar J, Gharebaghi Reza, Karimian Mehdi, Naseri Alireza, Sheikhi Mohammad
Department of Mycology, Pasteur Institute of Iran, Tehran, 13164, Iran.
Mycopathologia. 2006 Apr;161(4):245-9. doi: 10.1007/s11046-006-0222-7.
The effects of Akacid plus, a novel guanidine-based polymer first introduced as a biocidal and disinfectant agent were studied on Aspergillus parasiticus growth and its aflatoxin (AF) productivity. The fungus was cultured on yeast extract-sucrose (YES) broth in presence of various twofold serial dilutions of 25% Akacid plus (1.5-96 microL/50 mL medium) and then incubated in shaking condition with 150 rev./min at 28 degrees C for 96 h. Based on obtained results, Akacid plus was found to significantly inhibit both growth and aflatoxin B1 (AFB1) synthesis in very low concentrations in a dose-dependent manner. Fungal growth inhibition was determined in the range of 9.6-99.6% in mycelia exposed to the total concentration range of 1.5-48 microL. A final concentration of 96 microL was necessary to completely inhibit the growth of fungus. Under similar conditions, AFB1 synthesis was found to be strongly inhibited by 8.1-98.0% in presence of 1.5-24 microL Akacid plus with a maximum of 100% by 48 microL concentration. With respect to the unique physico-chemical properties of Akacid plus, its marked inhibitory effects on A. parasiticus growth and its AFB1 synthesis shown for the first time in this study make it a promising candidate for application in prevention programmes of AF contamination of susceptible crops.
研究了一种新型胍基聚合物Akacid plus作为杀菌剂和消毒剂首次使用时，对寄生曲霉生长及其黄曲霉毒素（AF）产生能力的影响。将该真菌在酵母提取物-蔗糖（YES）肉汤中培养，加入25% Akacid plus的各种两倍系列稀释液（1.5 - 96微升/50毫升培养基），然后在28℃、150转/分钟的振荡条件下孵育96小时。根据所得结果，发现Akacid plus在极低浓度下就能以剂量依赖方式显著抑制生长和黄曲霉毒素B1（AFB1）的合成。在暴露于1.5 - 48微升总浓度范围的菌丝体中，真菌生长抑制率在9.6% - 99.6%之间。完全抑制真菌生长需要96微升的终浓度。在类似条件下，发现1.5 - 24微升Akacid plus存在时，AFB1合成受到8.1% - 98.0%的强烈抑制，48微升浓度时最大抑制率达100%。鉴于Akacid plus独特的物理化学性质，本研究首次显示其对寄生曲霉生长及其AFB1合成具有显著抑制作用，这使其成为应用于易感作物AF污染预防计划的有前景的候选物。
