雌激素与催乳素对激素依赖性大鼠乳腺肿瘤的体外相互作用（38511）

In vitro interaction of estrogen and prolactin on hormone-dependent rat mammory tumors (38511).

作者信息

Lee C, Oyasu R, Chen C

出版信息

Proc Soc Exp Biol Med. 1975 Jan;148(1):224-6. doi: 10.3181/00379727-148-38511.

Abstract

Hormone-dependent, regressing mammary tumors in rats were incubated in a chemically defined culture medium containing tritium labeled leucine at 37 degrees for 2 hr. The rate of tritium incorporation into the protein fraction by these tissues was measured with and without the addition of 17beta-estradiol and/or ovine prolactin into the medium. Tumor tissue responded to either estrogen or porlactin by a 6-7% increase in the rate of 3-H-leucine incorporation. When the incubation medium contained both the hormones, the increase in the rate of 3-H leucine incorporation was 19% which was significantly greater than the sum of the effect of each single hormone measured independelntly. These results demonstrate a synergistic effect between estrogen and prolactin on rat mammary tumors.

摘要

将大鼠体内激素依赖性的退行性乳腺肿瘤置于含有氚标记亮氨酸的化学限定培养基中，于37℃孵育2小时。在培养基中添加和不添加17β - 雌二醇和/或羊催乳素的情况下，测定这些组织将氚掺入蛋白质组分的速率。肿瘤组织对雌激素或催乳素的反应是，3 - H - 亮氨酸掺入速率增加6 - 7%。当孵育培养基中同时含有这两种激素时，3 - H亮氨酸掺入速率的增加为19%，这显著大于单独测量的每种单一激素的效应之和。这些结果证明了雌激素和催乳素对大鼠乳腺肿瘤具有协同作用。