Shi Y, Seto E, Chang L S, Shenk T
Howard Hughes Medical Institute, Princeton University, New Jersey 08544-1014.
Cell. 1991 Oct 18;67(2):377-88. doi: 10.1016/0092-8674(91)90189-6.
A sequence within the transcription control region of the adeno-associated virus P5 promoter has been shown to mediate transcriptional activation by the adenovirus E1A protein. We report here that this same element mediates transcriptional repression in the absence of E1A. Two cellular proteins have been found to bind to overlapping regions within this sequence element. One of these proteins, YY1, is responsible for the repression. E1A relieves repression exerted by YY1 and further activates transcription through its binding site. A YY1-specific cDNA has been isolated. Its sequence reveals YY1 to be a zinc finger protein that belongs to the GLI-Krüppel gene family. The product of the cDNA binds to YY1 sites. When fused to the GAL4 DNA-binding domain, it is capable of repressing transcription directed by a promoter that contains GAL4-binding sites, and E1A proteins can relieve the repression and activate transcription through the fusion protein.
腺相关病毒P5启动子转录控制区域内的一个序列已被证明可介导腺病毒E1A蛋白的转录激活作用。我们在此报告，在没有E1A的情况下，同一元件介导转录抑制。已发现两种细胞蛋白与该序列元件内的重叠区域结合。其中一种蛋白YY1负责这种抑制作用。E1A可解除YY1施加的抑制，并通过其结合位点进一步激活转录。已分离出一个YY1特异性cDNA。其序列显示YY1是一种属于GLI-Krüppel基因家族的锌指蛋白。该cDNA的产物与YY1位点结合。当与GAL4 DNA结合结构域融合时，它能够抑制由含有GAL4结合位点的启动子指导的转录，并且E1A蛋白可以解除这种抑制并通过融合蛋白激活转录。
