干扰素可短暂调节红白血病Friend细胞中的核内信号系统。

Interferon transiently modulates intranuclear signalling system in erythroleukemia Friend cells.

作者信息

Miscia S, Cataldi A, Lisio R, Tulipano G, Rizzoli R, Rana R, Cocco L

机构信息

Istituto di Morfologia Umana Normale, Università di Chieti, Italy.

出版信息

Cell Biol Int Rep. 1991 May;15(5):427-35. doi: 10.1016/0309-1651(91)90130-b.

DOI:10.1016/0309-1651(91)90130-b

PMID:1655286

Abstract

The effect of human recombinant DNA interferon alpha type A on nuclear inositol lipids, diacylglycerol (DAG) and DNA metabolism has been investigated in Friend erythroleukemia cells. A transient enhancement of phosphatidylinositol (4,5) - bisphosphate (PIP2) phosphorylation together with an increase of diacylglycerol mass were observed in nuclei isolated from cells treated with interferon alpha for 90 min. At the same time, a marked reduction of DNA polymerase alpha activity was observed, suggesting a possible involvement of nuclear inositol fraction in the response of the cell nucleus to interferon treatment.

摘要

已在Friend红白血病细胞中研究了人重组DNAα型干扰素对细胞核肌醇脂质、二酰基甘油（DAG）和DNA代谢的影响。在用α干扰素处理90分钟的细胞分离出的细胞核中，观察到磷脂酰肌醇（4,5）-二磷酸（PIP2）磷酸化的短暂增强以及二酰基甘油质量的增加。同时，观察到DNA聚合酶α活性显著降低，这表明细胞核肌醇部分可能参与了细胞核对干扰素处理的反应。