Ren C S
Department of Molecular Biology, Cancer Research Institute of China Medical University, Shenyang.
Zhonghua Bing Li Xue Za Zhi. 1991 Jun;20(2):88-90.
Determination of p21, a product of Ha-ras oncogene, and HBsAg in hepatocellular carcinoma (HCC) and liver cirrhosis etc. was carried out with ABC method. The results showed that HCC tissues exhibited enhancement of p21 expression with a positive rate of 72.4%, which was obviously higher than the expression of p21 in tissues from liver cirrhosis, chronic hepatitis and hepatoblastoma. The p21 positive rate of regenerative cirrhosis nodules close to the HCC was 87.2%. The p21 expression level in HBsAg positive regenerative nodules of cirrhosis close to the HCC was significantly high, and its positive rate reached 93.9%. The expression level of p21 protein in well-differentiated HCC was higher than that of poorly differentiated and undifferentiated HCC. Therefore, the result suggests that the expression level of p21 in liver cirrhosis is related to persistent infection of HBV. The elevated expression of p21 plays an important role in the development of regenerative nodules in liver cirrhosis towards HCC, and it is also an important factor in the early stage of HCC development.
采用ABC法对肝癌（HCC）、肝硬化等组织中的Ha-ras癌基因产物p21和乙肝表面抗原（HBsAg）进行检测。结果显示，肝癌组织中p21表达增强，阳性率为72.4%，明显高于肝硬化、慢性肝炎及肝母细胞瘤组织中p21的表达。靠近肝癌的再生性肝硬化结节中p21阳性率为87.2%。靠近肝癌的肝硬化HBsAg阳性再生结节中p21表达水平显著升高，阳性率达93.9%。高分化肝癌中p21蛋白表达水平高于低分化及未分化肝癌。因此，结果提示肝硬化中p21的表达水平与乙肝病毒持续感染有关。p21表达升高在肝硬化再生结节向肝癌发展过程中起重要作用，也是肝癌发生早期的重要因素。
