Bron P A, Molenaar D, de Vos W M, Kleerebezem M
Wageningen Centre for Food Sciences, Wageningen, the Netherlands.
J Appl Microbiol. 2006 Apr;100(4):728-38. doi: 10.1111/j.1365-2672.2006.02891.x.
The purpose of this study was to determine the global transcriptional response in a food-associated lactic acid bacterium during bile stress.
Clone-based DNA micro-arrays were employed to describe the global transcriptional response of Lactobacillus plantarum WCFS1 towards 0.1% porcine bile. Comparison of differential transcript profiles obtained during growth of Lact. plantarum on plates with and without bile revealed 28 and 62 putative genes, of which the expression was at least 2.5-fold up- or down-regulated by bile, respectively. Approximately, 50% of these genes appeared genetically linked, and 12 bile-responsive gene clusters were identified. Seven of the identified bile-responsive genes and gene clusters encode typical stress-related functions, including glutathione reductase and glutamate decarboxylase, involved in oxidative and acid stress, respectively. Moreover, 14 bile-responsive genes and gene clusters were identified that encode proteins that are located in the cell envelope, including the dlt operon and the F1F0 ATPase.
The identification of a relatively high number of genes encoding cell envelope functions indicates a major impact of bile acids on the integrity and/or functionality of the cytoplasmic membrane and cell wall.
The data presented here provide valuable clues towards the defence mechanisms that play a role during bile stress in Lact. plantarum.
本研究的目的是确定一种与食品相关的乳酸菌在胆汁应激期间的全局转录反应。
采用基于克隆的DNA微阵列来描述植物乳杆菌WCFS1对0.1%猪胆汁的全局转录反应。比较植物乳杆菌在有胆汁和无胆汁平板上生长期间获得的差异转录谱，发现了28个和62个推定基因，其表达分别被胆汁上调或下调至少2.5倍。这些基因中约50%在遗传上似乎是连锁的，并鉴定出12个胆汁反应基因簇。鉴定出的7个胆汁反应基因和基因簇编码典型的应激相关功能，包括分别参与氧化应激和酸应激的谷胱甘肽还原酶和谷氨酸脱羧酶。此外，还鉴定出14个胆汁反应基因和基因簇，它们编码位于细胞膜上的蛋白质，包括dlt操纵子和F1F0 ATP酶。
鉴定出相对大量编码细胞膜功能的基因表明胆汁酸对细胞质膜和细胞壁的完整性和/或功能有重大影响。
此处呈现的数据为植物乳杆菌在胆汁应激期间发挥作用的防御机制提供了有价值的线索。
Zotero 插件