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以米泔水水解蜗牛肉作为动物饲料的新型益生菌补充剂

Hydrolyzation of snail meat with rice water as novel probiotic supplements for animal feed.

作者信息

Suryadi Ujang, Hertamawati Rosa Tri, Imam And Shokhirul

机构信息

Department of Animal Science, Politeknik Negeri Jember, Mastrip Street PO. BOX 164, Jember, East Java, Indonesia.

出版信息

Vet World. 2022 Apr;15(4):937-942. doi: 10.14202/vetworld.2022.937-942. Epub 2022 Apr 15.

Abstract

BACKGROUND AND AIM

Snail meat and digestive tract hydrolyzate fermented with a consortium of preserved rice water microorganisms could serve as new sources of probiotics. Microorganisms from the examined feed supplement were isolated, identified, and characterized for resistance at low pH and with bile salts. The study aimed to determine the potential hydrolysate of the snail meat and digestive tract as a novel probiotic supplement for animal feed at various pH values and Oxgall.

MATERIALS AND METHODS

The submerged fermentation method was conducted for 21 days to examine the novel probiotic that originated from snail microorganisms in the hydrolyzed liquid fermented by finely ground snail meat and the digestive tract. The microorganisms in the hydrolyzate were isolated by a spread plate method, while the potential of the probiotic hydrolyzate was tested for resistance to pH values of 2, 2.5, 3, and 4, as well as resistance to bile salts at Oxgall concentrations of 0.2%, 0.3%, 0.5%, 1%, and 2%.

RESULTS

The hydrolyzate profile of snail meat and digestive tract contained five isolates of lactic acid bacteria that could serve as potential probiotics.

CONCLUSION

The application of fermentation technology using a consortium of preserved rice water microorganisms can convert snail meat and the digestive tract into novel probiotic products that could be utilized in feed supplements.

摘要

背景与目的

用淘米水微生物菌群发酵的蜗牛肉和消化道水解物可作为新的益生菌来源。从受试饲料添加剂中分离、鉴定微生物,并对其在低pH值和胆盐环境下的抗性进行表征。本研究旨在确定蜗牛肉和消化道的潜在水解物作为一种新型益生菌补充剂在不同pH值和牛胆汁条件下用于动物饲料的可能性。

材料与方法

采用深层发酵法进行21天,以检测由精细研磨的蜗牛肉和消化道在水解液中发酵产生的新型益生菌。通过平板涂布法分离水解物中的微生物,同时测试益生菌水解物对pH值2、2.5、3和4的抗性,以及对牛胆汁浓度为0.2%、0.3%、0.5%、1%和2%的胆盐的抗性。

结果

蜗牛肉和消化道的水解物谱含有五种乳酸菌分离株,可作为潜在的益生菌。

结论

利用淘米水微生物菌群的发酵技术可将蜗牛肉和消化道转化为可用于饲料添加剂的新型益生菌产品。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b72/9178601/dfac69a6eea0/Vetworld-15-937-g001.jpg

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