来自长双歧杆菌益生菌株的免疫刺激DNA序列在体外抑制IgE产生。
An immunostimulatory DNA sequence from a probiotic strain of Bifidobacterium longum inhibits IgE production in vitro.
作者信息
Takahashi Noritoshi, Kitazawa Haruki, Shimosato Takeshi, Iwabuchi Noriyuki, Xiao Jin-Zhong, Iwatsuki Keiji, Kokubo Sadayuki, Saito Tadao
机构信息
Food Research and Development Laboratory, Morinaga Milk Industry Co., Ltd, Zama, Kanagawa, Japan.
出版信息
FEMS Immunol Med Microbiol. 2006 Apr;46(3):461-9. doi: 10.1111/j.1574-695X.2006.00064.x.
The immunostimulatory oligodeoxynucleotide (ODN) BL07 (5'-GCGTCGGTTTCGGTGCTCAC-3') was identified from the genomic DNA of the probiotic strain Bifidobacterium longum BB536. ODN BL07 stimulated B-lymphocyte proliferation and induced interleukin-12 (IL-12) production in macrophage-like J774.1 cells. ODNs BL07 and BL07S (modified with phosphorothioate backbone) significantly inhibited immunoglobulin E (IgE) production and stimulated interferon-gamma (IFN-gamma) and IL-12 production, but did not affect IL-4 secretion in murine splenic cells of ovalbumin-primed BALB/c mice. These ODNs also significantly inhibited production of IgE in purified murine B cells in the presence of IL-4 and anti-CD40. The results suggest the potential of ODNs BL07 and BL07S in preventing IgE-related immune responses and the possible involvement of ODN BL07 in the antiallergic efficacy of B. longum BB536.
免疫刺激寡脱氧核苷酸（ODN）BL07（5'-GCGTCGGTTTCGGTGCTCAC-3'）是从益生菌长双歧杆菌BB536的基因组DNA中鉴定出来的。ODN BL07刺激B淋巴细胞增殖，并在巨噬细胞样J774.1细胞中诱导白细胞介素-12（IL-12）的产生。ODN BL07和BL07S（用硫代磷酸酯骨架修饰）显著抑制免疫球蛋白E（IgE）的产生，并刺激干扰素-γ（IFN-γ）和IL-12的产生，但不影响卵清蛋白致敏的BALB/c小鼠脾细胞中IL-4的分泌。在存在IL-4和抗CD40的情况下，这些ODN也显著抑制纯化的小鼠B细胞中IgE的产生。结果表明ODN BL07和BL07S在预防IgE相关免疫反应方面的潜力，以及ODN BL07可能参与长双歧杆菌BB536的抗过敏功效。
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