Division of Infectious Diseases, National Health Research Institutes, Miaoli, Taiwan, ROC.
Int Arch Allergy Immunol. 2011;156(2):128-36. doi: 10.1159/000322350. Epub 2011 May 16.
Beneficial effects of probiotics have been reported for patients with allergic diseases and intestinal disorders. There is increasing interest in studying the role of different strains or combined probiotic administration on immunoregulation. In this study, we investigated whether probiotics modulate the immune response through regulating T cell proliferation and differentiation.
We examined the effect of probiotic I (a combination of Lactobacillus acidophilus and Bifidobacterium bifidus) and probiotic II (a combination of L. acidophilus and B. infantis) on cell survival and proliferation, the progression of the cell cycle, and the production of Th1/Th2 cytokines by mitogen-stimulated murine spleen cells and human peripheral blood mononuclear cells (PBMCs).
Our experimental results showed that high concentrations (≥ 1 × 10(6) CFU/ml) of probiotic I or II inhibited mitogen-induced cell proliferation and arrested the cell cycle at the G0/G1 stage in both mitogen-stimulated spleen cells and PBMCs. In the results of low concentrations (<1 × 10(6) CFU/ml), probiotic I or II enhanced the production of IFN-γ but inhibited the production of IL-4. Our results indicated that high concentrations of probiotic I or II treatment could attenuate mitogen-induced overactive immune responses. On the other hand, low concentrations of probiotic I or II treatment could promote a shift in the Th1/Th2 balance toward Th1-skewed immunity.
Dose selection is an important issue for probiotic studies. Our results indicated that probiotics have beneficial effects on regulating T cell-mediated immune responses by attenuating mitogen-induced overactive immune responses and promoting Th1 immune responses.
益生菌对过敏性疾病和肠道疾病患者具有有益作用。人们越来越关注研究不同菌株或联合益生菌给药对免疫调节的作用。在这项研究中，我们研究了益生菌是否通过调节 T 细胞增殖和分化来调节免疫反应。
我们研究了益生菌 I（嗜酸乳杆菌和双歧杆菌的组合）和益生菌 II（嗜酸乳杆菌和婴儿双歧杆菌的组合）对有丝分裂原刺激的鼠脾细胞和人外周血单核细胞（PBMC）中细胞存活和增殖、细胞周期进程以及 Th1/Th2 细胞因子产生的影响。
我们的实验结果表明，高浓度（≥1×10(6)CFU/ml）的益生菌 I 或 II 抑制有丝分裂原诱导的细胞增殖，并使有丝分裂原刺激的脾细胞和 PBMC 中的细胞周期停滞在 G0/G1 期。在低浓度（<1×10(6)CFU/ml）下，益生菌 I 或 II 增强 IFN-γ的产生，但抑制 IL-4 的产生。我们的结果表明，高浓度的益生菌 I 或 II 处理可以减弱有丝分裂原诱导的过度活跃的免疫反应。另一方面，低浓度的益生菌 I 或 II 处理可以促进 Th1/Th2 平衡向 Th1 优势免疫的转变。
剂量选择是益生菌研究的一个重要问题。我们的结果表明，益生菌通过减弱有丝分裂原诱导的过度活跃的免疫反应和促进 Th1 免疫反应，对调节 T 细胞介导的免疫反应具有有益作用。
