Thenmozhi V, Rajendran R, Ayanar K, Manavalan R, Tyagi B K
Centre for Research in Medical Entomology, Madurai, India.
Trop Med Int Health. 2006 Mar;11(3):288-93. doi: 10.1111/j.1365-3156.2006.01567.x.
To investigate the role of Anopheles subpictus Grassi as a vector of Japanese encephalitis virus (JEV) transmission in Cuddalore, an area of Tamil Nadu endemic for the disease.
We collected 98 pools (4,900 specimens) of wild adult male An. subpictus mosquitoes outdoors during dusk hours and screened them for JEV antigen by antigen-capture Enzyme Linked Immunosorbent Assay. Additionally, over a period of 1 year, we tested 166 pools (8,300 specimens) of wild adult female An. subpictus mosquitoes collected indoors for JEV.
Four pools of male An. subpictus tested positive. This indicates possible natural transovarial transmission of the virus through An. subpictus. Nineteen female pools were positive with a minimum infection rate of 2.3. From January through March the maximum infection rate was highest: 5.0 compared with 1.7 between April and September and 2.1 from October to December, although the difference was not statistically significant. From the 19 positive female pools, four isolates were confirmed as JEV by insect bioassay.
The role of An. subpictus as a secondary vector in JEV transmission in Cuddalore, Tamil Nadu lends support to the hypothesis of periodic epidemics in the region.
在泰米尔纳德邦库德洛尔地区（该疾病的地方性流行区），研究伪杂鳞库蚊作为日本脑炎病毒（JEV）传播媒介的作用。
我们在黄昏时分于户外收集了98组（4900只样本）野生成年雄性伪杂鳞库蚊，通过抗原捕获酶联免疫吸附测定法对其进行JEV抗原筛查。此外，在1年的时间里，我们对在室内收集的166组（8300只样本）野生成年雌性伪杂鳞库蚊进行了JEV检测。
4组雄性伪杂鳞库蚊检测呈阳性。这表明该病毒可能通过伪杂鳞库蚊进行自然经卵传播。19组雌性库蚊呈阳性，最低感染率为2.3。从1月到3月，最高感染率最高：为5.0，而4月至9月为1.7，10月至12月为2.1，尽管差异无统计学意义。在19组呈阳性的雌性库蚊中，通过昆虫生物测定法确认有4株分离株为JEV。
在泰米尔纳德邦库德洛尔地区，伪杂鳞库蚊作为JEV传播的次要媒介，支持了该地区周期性流行的假说。
