来自菲律宾打拉省蚊子的日本脑炎病毒III型。
Japanese encephalitis virus genotype III from mosquitoes in Tarlac, Philippines.
作者信息
Aure Wilfredo E, Sayama Yusuke, Saito-Obata Mariko, Salazar Nelia P, Malbas Fedelino F, Galang Hazel O, Imamura Tadatsugu, Zuasula Cecilia L, Oshitani Hitoshi
机构信息
Department of Medical Entomology, Research Institute for Tropical Medicine, Filinvest Corporate City, Alabang, Muntinlupa City, Metro Manila, Philippines, 1781.
Universiti Malaysia Sabah, Faculty of Medicine and Health Sciences, Kota Kinabalu, Sabah, Malaysia.
出版信息
IJID Reg. 2022 May 16;4:59-65. doi: 10.1016/j.ijregi.2022.05.005. eCollection 2022 Sep.
OBJECTIVES
The aim of this study was to investigate the presence of Japanese encephalitis virus (JEV) in a rice-farming community in the Philippines and to determine its implications regarding the epidemiology of viral encephalitides in the Asia-Pacific Region.
METHODS
Mosquitoes were collected monthly from animal-baited traps close to flooded rice fields in two barangays (villages) in the Municipality of San Jose, Tarlac Province in Luzon, from May 2009 to July 2010. Virus was detected by nested reverse transcription PCR. Phylogenetic analysis of the amplified virus envelope gene was done using the maximum-likelihood method.
RESULTS
A total of 28 700 known vector mosquitoes were collected, namely , and . JEV genotype III was detected in , belonging to the same genotype but form a different clade from those reported in the 1980s and in 2020 in this country.
CONCLUSIONS
Japanese encephalitis is associated with rice cultivation and the presence of infected mosquitoes in Tarlac, Philippines. It remains to be seen whether the observed genetic shift of genotype III to genotype I in Asia will in time have an impact on the epidemiology of Japanese encephalitis in the Philippines. For long-term disease control, regular surveillance and Japanese encephalitis immunization in children and travelers in high risk areas are recommended.
目的
本研究旨在调查菲律宾一个水稻种植社区中日本脑炎病毒（JEV）的存在情况，并确定其对亚太地区病毒性脑炎流行病学的影响。
方法
2009年5月至2010年7月，每月在吕宋岛打拉省圣何塞市两个 barangays（村庄）靠近水淹稻田的动物诱饵诱捕器中收集蚊子。通过巢式逆转录PCR检测病毒。使用最大似然法对扩增的病毒包膜基因进行系统发育分析。
结果
共收集了28700只已知的媒介蚊子，即 ， 和 。在 中检测到JEV基因型III，属于同一基因型，但与该国20世纪80年代和2020年报告的基因型形成不同的进化枝。
结论
日本脑炎与菲律宾打拉省的水稻种植和感染蚊子的存在有关。亚洲观察到的基因型III向基因型I的基因转变是否会及时对菲律宾的日本脑炎流行病学产生影响，仍有待观察。为了长期控制疾病，建议对高危地区的儿童和旅行者进行定期监测和日本脑炎免疫接种。
Zotero 插件