Malimas Taweesak, Yukphan Pattaraporn, Takahashi Mai, Potacharoen Wanchern, Tanasupawat Somboon, Nakagawa Yasuyoshi, Tanticharoen Morakot, Yamada Yuzo
BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand.
Biosci Biotechnol Biochem. 2006 Mar;70(3):684-90. doi: 10.1271/bbb.70.684.
Twenty-three strains, which were assigned to Gluconobacter frateurii and maintained at Culture Collection NBRC, were re-identified at the species level on the basis of restriction analysis of 16S-23S rDNA ITS regions by digestion with six restriction endonucleases: Bsp1286I, MboII, AvaII, TaqI, BsoBI, and BstNI. The strains examined were divided into six groups, Group III-1, Group III-2, Group III-3, Group III-4, Group III-5, and Group IV. Group III-1 and Group III-4 respectively were divided into two subgroups, Subgroup III-1a, Subgroup III-1b and Subgroup III-4a, Subgroup III-4b. Gluconobacter frateurii NBRC 3264(T) was included in Group III-2, along with strains NBRC 3265 and NBRC 3270, and G. thailandicus BCC 14116(T) was included in Group III-3, along with strains NBRC 3254, NBRC 3256, NBRC 3258, NBRC 3255, and NBRC 3257. These groupings were supported by a phylogenetic tree based on 16S-23S rDNA ITS sequences. Strains of group III-2 and Group IV were unequivocally re-identified as G. frateurii, but strains of Group III-3, Group III-4, and Group III-5 were not necessarily re-identified as G. frateurii. The results obtained indicate that the 23 strains have a taxonomically heterogeneous nature, and they are referred to as the G. frateurii complex.
23株被归类为弗氏葡萄糖杆菌并保藏于日本技术评价研究所生物资源中心（NBRC）的菌株，基于用六种限制性内切酶（Bsp1286I、MboII、AvaII、TaqI、BsoBI和BstNI）对16S - 23S rDNA ITS区域进行限制性分析，在种水平上进行了重新鉴定。所检测的菌株被分为六组，即III - 1组、III - 2组、III - 3组、III - 4组、III - 5组和IV组。III - 1组和III - 4组又分别分为两个亚组，即III - 1a亚组、III - 1b亚组和III - 4a亚组、III - 4b亚组。弗氏葡萄糖杆菌NBRC 3264(T)与菌株NBRC 3265和NBRC 3270一起被归入III - 2组，泰国葡萄糖杆菌BCC 14116(T)与菌株NBRC 3254、NBRC 3256、NBRC 3258、NBRC 3255和NBRC 3257一起被归入III - 3组。基于16S - 23S rDNA ITS序列构建的系统发育树支持了这些分组。III - 2组和IV组的菌株被明确重新鉴定为弗氏葡萄糖杆菌，但III - 3组、III - 4组和III - 5组的菌株不一定能重新鉴定为弗氏葡萄糖杆菌。所得结果表明这23株菌株具有分类学上的异质性，它们被称为弗氏葡萄糖杆菌复合体。
