Lan Huong Vu Thi, Malimas Taweesak, Yukphan Pattaraporn, Potacharoen Wanchern, Tanasupawat Somboon, Thanh Loan Ly Thi, Tanticharoen Morakot, Yamada Yuzo
BIOTEC Culture Collection, BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
J Gen Appl Microbiol. 2007 Apr;53(2):133-42. doi: 10.2323/jgam.53.133.
Forty-four Thai isolates phenotypically assigned to the genus Gluconobacter were examined for 16S-23S rDNA ITS restriction analysis by MboII and SduI (=Bsp1286I) digestions. The Thai isolates tested were divided into seven groups: Group I for fourteen isolates, Group IX for one isolate, Group X for two isolates, Group V-2 for four isolates, Group XI for three isolates, Group IV for one isolate, and Group III for nineteen isolates. There were no isolates of either Group II or Group V-1 that were identified as G. cerinus. The isolates of Group III, Group IV, and Group XI were subjected to an additional 16S-23S rDNA ITS restriction analysis by AvaII, TaqI, BsoBI, and BstNI digestions. The isolates of Group III were divided into three groups and two subgroups: Group III-2 for five isolates, Group III-6 for two isolates, and Group III-4, which was divided into two subgroups, Subgroup III-4a for four isolates and Subgroup III-4b for eight isolates. The fourteen isolates of Group I were identified as G. oxydans, and the two isolates of Group X were temporarily identified as G. oxydans. The five isolates of Group III-2 and the one isolate of Group IV were identified as G. frateurii. The remaining twenty-two isolates of Group V-2, Group III-4, Group III-6, Group IX, and Group XI were not identified but are candidates for several new species.
对44株表型归为葡糖杆菌属的泰国分离株进行了16S-23S rDNA ITS限制性分析，采用MboII和SduI（=Bsp1286I）酶切。所检测的泰国分离株分为七组：I组有14株分离株，IX组有1株分离株，X组有2株分离株，V-2组有4株分离株，XI组有3株分离株，IV组有1株分离株，III组有19株分离株。未鉴定出II组或V-1组中有被鉴定为产黄葡糖杆菌的分离株。对III组、IV组和XI组的分离株进行了额外的16S-23S rDNA ITS限制性分析，采用AvaII、TaqI、BsoBI和BstNI酶切。III组的分离株分为三组和两个亚组：III-2组有5株分离株，III-6组有2株分离株，III-4组分为两个亚组，III-4a亚组有4株分离株，III-4b亚组有8株分离株。I组的14株分离株被鉴定为氧化葡糖杆菌，X组的2株分离株暂被鉴定为氧化葡糖杆菌。III-2组的5株分离株和IV组的1株分离株被鉴定为弗氏葡糖杆菌。V-2组、III-4组、III-6组、IX组和XI组的其余22株分离株未被鉴定，但可能是几个新物种的候选菌株。
