Hazard Kristina, Eliasson Linda, Dillner Joakim, Forslund Ola
Department of Laboratory Medicine, Division of Medical Microbiology, Lund University, University Hospital, Malmö, Sweden.
Int J Cancer. 2006 Sep 1;119(5):1073-7. doi: 10.1002/ijc.21920.
The human papillomaviruses (HPVs) exist as more than 100 distinct types. While variants of HPV are common, only few HPV subtypes have been reported. HPV type 38 has been proposed to be associated with nonmelanoma skin cancer (NMSC), with reported prevalences of up to 55%. A subtype of HPV38 was cloned, completely sequenced and found to have a 96% sequence similarity to prototype HPV38 in the L1 open reading frame. The presence of prototype HPV38 and HPV38b[FA125] was examined in paired biopsies of tape-stripped skin lesions and healthy skin from 269 immunocompetent patients by real-time PCR. Prototype HPV38 and HPV38b[FA125] were present in seven (3%) and five (2%) lesions, respectively, in viral loads ranging from one copy per 150 cells to one copy per 70,000 cells. In summary, we found that HPV38 is heterogeneous and is one of so far only few HPVs that contain subtypes. The heterogeneity needs to be considered in studies of the biology of this virus.
人乳头瘤病毒（HPV）有100多种不同类型。虽然HPV变异很常见，但只有少数HPV亚型被报道过。有人提出HPV 38型与非黑色素瘤皮肤癌（NMSC）有关，报道的患病率高达55%。一种HPV38亚型被克隆、完全测序，发现在L1开放阅读框中与原型HPV38有96%的序列相似性。通过实时PCR检测了269名免疫功能正常患者的胶带剥离皮肤病变和健康皮肤的配对活检样本中原型HPV³⁸和HPV38b[FA125]的存在情况。原型HPV38和HPV38b[FA125]分别存在于7个（3%）和5个（2%）病变中，病毒载量范围从每150个细胞1个拷贝到每70,000个细胞1个拷贝。总之，我们发现HPV38具有异质性，是迄今为止仅有的少数含有亚型的HPV之一。在对这种病毒的生物学研究中需要考虑到这种异质性。
