Alotaibi Laila, Provost Nathalie, Gagnon Simon, Franco Eduardo L, Coutlée François
Laboratoire de Virologie Moléculaire, Centre de Recherche, Département de Microbiologie et Infectiologie, et Medecine, Centre Hospitalier de l'Université de Montréal, Montréal, Que., Canada H2L 4M1.
J Clin Virol. 2006 Jun;36(2):133-40. doi: 10.1016/j.jcv.2006.02.007. Epub 2006 May 5.
Human papillomavirus (HPV) is ubiquitous on the skin of normal and immunosuppressed hosts.
We describe the diversity of HPV types in skin specimens using PCR-sequencing directly and after cloning with FAP59/64 or HVP2/B5 primers.
Cross-sectional analysis of skin swabs.
Seventy-five (92.6%) of 81 subjects provided samples that could be analysed with PCR (34 healthy controls <50 years old, 13 healthy controls > or =50 years old, 12 with actinic keratosis (AK), 8 with squamous cell carcinoma (SCC) and 8 renal transplant recipients). HPV DNA was detected more frequently with FAP59/64 (68/75, 91%) than with HVP2/B5 (9/75, 12%) (p<0.001). Agreement of typing results using FAP59/64 primers with both sequencing strategies was fair (mean kappa 0.56+/-0.19, 95% CI: 0.46-0.65). HPV species 1 and 2 of the beta-papillomavirus genus were associated with the presence of AK (OR=24.8, 95% CI: 2.3-262.6). A greater number of HPV types per sample was found in individuals with AK or SCC (p=0.046) or AK alone (p=0.02), than in healthy participants.
HPV infection on the skin is best evaluated with a combination of primers and sequencing strategies. Novel putative types were frequently detected in SCC. Skin lesions have a greater number of HPV types than normal skin.
人乳头瘤病毒（HPV）在正常宿主和免疫抑制宿主的皮肤上普遍存在。
我们通过直接PCR测序以及使用FAP59/64或HVP2/B5引物克隆后，描述皮肤标本中HPV类型的多样性。
皮肤拭子的横断面分析。
81名受试者中有75名（92.6%）提供了可用于PCR分析的样本（34名年龄小于50岁的健康对照者，13名年龄大于或等于50岁的健康对照者，12名患有光化性角化病（AK）者，8名患有鳞状细胞癌（SCC）者以及8名肾移植受者）。使用FAP59/64检测HPV DNA的频率（68/75，91%）高于使用HVP2/B5（9/75，12%）（p<0.001）。使用FAP59/64引物的分型结果与两种测序策略的一致性一般（平均kappa值0.56±0.19，95%可信区间：0.46 - 0.65）。β-乳头瘤病毒属的HPV 1型和2型与AK的存在相关（比值比=24.8，95%可信区间：2.3 - 262.6）。与健康参与者相比，患有AK或SCC的个体（p=0.046）或仅患有AK的个体（p=0.02）每个样本中发现的HPV类型更多。
皮肤HPV感染最好通过引物和测序策略相结合来评估。在SCC中经常检测到新的假定类型。皮肤病变中的HPV类型比正常皮肤更多。
