Department of Animal Diseases, University of Connecticut, Storrs, Connecticut 06268.
Infect Immun. 1970 Jul;2(1):101-4. doi: 10.1128/iai.2.1.101-104.1970.
Sixteen bovine genital mycoplasmal isolates obtained from semen and prepuce of bulls and from aborted fetuses were compared physiologically and serologically with the Donetta strain (tentatively Mycoplasma agalactiae var. bovis), a known pathogen. All isolates were distinct from the Donetta organism. Four appeared to be saprophytes, and the remainder were placed in one group which could not be further separated by the biochemical or serological methods used. Two of the organisms in the latter group have been subsequently identified as M. bovigenitalium. Uterine infusion of broth cultures of four isolates into virgin heifers failed to produce clinical evidence of disease, and significant lesions were not present at necropsy. The mycoplasmas were recovered from cervicovaginal mucus of only three heifers, and never for more than 3 days postinfusion. Since the organisms were not recovered from any organs at necropsy, it appears that the mycoplasmas were incapable of surviving in the clinically normal virgin female reproductive tract.
从公牛精液和包皮以及流产胎儿中获得的 16 株牛生殖道支原体分离物与已知病原体 Donetta 株（暂定支原体牛亚种）在生理和血清学上进行了比较。所有分离物均与 Donetta 菌不同。其中 4 株似乎是腐生菌，其余分离物被归入一组，无法通过使用的生化或血清学方法进一步分离。后一组中的两种生物随后被鉴定为 M. bovigenitalium。将 4 株分离物的肉汤培养物经子宫内注入到处女小母牛体内，未能产生疾病的临床证据，剖检时也没有明显病变。只有 3 头小母牛的宫颈阴道黏液中能回收支原体，且仅能在接种后 3 天内回收。由于在剖检时没有从任何器官中回收到这些生物体，因此似乎这些支原体无法在临床上正常的处女雌性生殖道中存活。
