Department of Animal Diseases, University of Connecticut, Storrs, Connecticut 06268.
Infect Immun. 1970 Jul;2(1):105-11. doi: 10.1128/iai.2.1.105-111.1970.
An electron microscopic examination of the pathogenic Donetta strain mycoplasma (tentatively Mycoplasma agalactiae var. bovis), in broth and cell cultures, revealed that this organism possessed considerable pleomorphism. A membranelined space occurred in a number of the organisms, and some of these were considered to represent deep, cuplike invaginations of the outer limiting membrane. In cell cultures, the mycoplasmas were located almost exclusively in extracellular locations but often in close association to the host-cell membrane. The cytopathic effects (CPE) were not considered to be caused primarily by cytoplasmic invasion. Serum was necessary for the development of the CPE and multiplication of the mycoplasmas in cell cultures. An effort to determine the basic cause of the CPE was unsuccessful but did eliminate causes previously reported for other strains of mycoplasma.
电子显微镜检查致病性 Donetta 株支原体（暂定牛支原体亚种）在肉汤和细胞培养物中的形态，发现该生物体具有相当大的多形性。许多生物体中出现了膜衬空间，其中一些被认为代表了外限膜的深杯状内陷。在细胞培养物中，支原体几乎完全位于细胞外位置，但通常与宿主细胞膜密切相关。细胞病变效应（CPE）不被认为主要是由细胞质入侵引起的。血清对于细胞培养物中 CPE 的发展和支原体的增殖是必要的。试图确定 CPE 的基本原因的努力没有成功，但排除了以前报道的其他支原体菌株的原因。
