金黄色葡萄球菌肠毒素 B 产生的趋化活性。
Chemotactic activity generated by staphylococcal enterotoxin B.
机构信息
U. S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701.
出版信息
Infect Immun. 1971 Jan;3(1):94-9. doi: 10.1128/iai.3.1.94-99.1971.
Staphylococcal enterotoxin B (SEB) has significant toxicity for several mammalian species by both oral and parenteral routes. When highly purified SEB was incubated for 1 hr at 37 C with fresh serum from normal young adult men with little or no antibody activity for SEB, a factor(s) chemotactic for human polymorphonuclear leukocytes (PMN) was formed. Similar experiments using rabbit serum and autologous peritoneal PMN also demonstrated chemotactic activation. All chemotactic activity generated at 37 C was destroyed by heating at 56 C for 30 min. In human studies, precipitating antibody to SEB prevented generation of chemotactic activity by SEB. Based on heat lability and antibody sensitivity, the chemotactic factor(s) generated by SEB differs from that generated by endotoxin, and suggests a mechanism by which the PMN may participate in the pathophysiology of enterotoxemia.
金黄色葡萄球菌肠毒素 B（SEB）通过口服和注射途径对多种哺乳动物具有显著毒性。当高纯度 SEB 在 37°C 下与来自正常年轻成年男性的新鲜血清孵育 1 小时，这些血清对 SEB 的抗体活性很少或没有时，会形成一种趋化因子（s），可趋化人多形核白细胞（PMN）。使用兔血清和自身腹腔 PMN 的类似实验也证明了趋化活性的激活。在 37°C 下产生的所有趋化活性都在 56°C 加热 30 分钟后被破坏。在人体研究中，针对 SEB 的沉淀抗体可防止 SEB 产生趋化活性。基于热不稳定性和抗体敏感性，SEB 产生的趋化因子（s）与内毒素产生的趋化因子不同，这表明 PMN 可能参与肠毒素血症的病理生理学过程。
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