Comparative Pathology Section, Laboratory Aids Branch, Division of Research Services, National Institutes of Health, Bethesda, Maryland 20014.
Infect Immun. 1971 Mar;3(3):429-37. doi: 10.1128/iai.3.3.429-437.1971.
Bacillus piliformis (Tyzzer) was isolated from the liver of rabbits with Tyzzer's disease and serially passaged in embryonated hens' eggs. Weanling rabbits given the 32nd egg passage developed lesions typical of Tyzzer's disease and died. B. piliformis was reisolated from the liver of these rabbits in embryonated eggs. Outside the host cell, the motile vegetative phase appeared to be unstable, and no means was found to preserve its viability; the results of titrations were believed to be dependent upon the resistant stage or spore. The spore withstood repeated freeze and thaw and was resistant to heat treatment of 56 C for 1 hr but not 80 C for 0.5 hr. None of several antibacterial substances tested in embryonated eggs was completely inhibitory; B. piliformis was resistant to sulfamethazine and chloramphenicol. The taxonomic position of this pleomorphic, gram-negative, sporeforming, pathogenic bacterium which appears to grow only in certain cells of several species remains unresolved.
从患有 Tyzzer 病的兔子的肝脏中分离出了短杆菌（Tyzzer），并在鸡胚中进行了连续传代。给予第 32 代卵传代的断奶兔出现了 Tyzzer 病的典型病变并死亡。从这些兔子的肝脏中在鸡胚中重新分离出了短杆菌。在宿主细胞外，运动的营养阶段似乎不稳定，并且没有找到保存其活力的方法；滴定的结果被认为取决于抗性阶段或孢子。孢子能经受住反复的冷冻和解冻，并且能耐受 56°C 加热 1 小时，但不能耐受 80°C 加热 0.5 小时。在鸡胚中测试的几种抗菌物质都不完全抑制；短杆菌对磺胺甲噁唑和氯霉素有抗性。这种多形的、革兰氏阴性的、孢子形成的、致病性细菌的分类位置仍然没有解决，这种细菌似乎只在几个物种的某些细胞中生长。
