Weiss E
Department of Microbiology, Naval Medical Research Institute, Bethesda, Maryland 20014.
J Bacteriol. 1970 Jan;101(1):133-7. doi: 10.1128/jb.101.1.133-137.1970.
When resting cells of Neisseria meningitidis group B were incubated with either succinate, fumarate, or malate, respiration and CO(2) production were not significantly stimulated. These dicarboxylic acids were readily utilized, however, when they were added in association with a combination of alpha-ketoglutarate and aspartate or with glucose or with glutamate. The amounts of these substrates required for exogenous succinate utilization were relatively large. Both the alpha-ketoglutarate-aspartate combination and glutamate greatly stimulated succinate uptake into the cells, but glutamate was far more effective than the alpha-ketoglutarate-aspartate combination in eliciting exogenous succinate utilization. This difference is explained on the basis of evidence reported in another article that succinate derived from the alpha-ketoglutarate-aspartate mixture is metabolized more rapidly-and thus more rapidly dilutes the specific activity of added succinate-than the succinate derived from glutamate.
当将B群脑膜炎奈瑟菌的静息细胞与琥珀酸、富马酸或苹果酸一起孵育时，呼吸作用和二氧化碳生成并未受到显著刺激。然而，当这些二羧酸与α-酮戊二酸和天冬氨酸的组合、或与葡萄糖、或与谷氨酸一起添加时，它们很容易被利用。外源利用琥珀酸所需的这些底物量相对较大。α-酮戊二酸-天冬氨酸组合和谷氨酸都极大地刺激了琥珀酸摄入细胞，但在引发外源琥珀酸利用方面，谷氨酸比α-酮戊二酸-天冬氨酸组合有效得多。另一篇文章报道的证据解释了这种差异，即α-酮戊二酸-天冬氨酸混合物衍生的琥珀酸比谷氨酸衍生的琥珀酸代谢更快，因此能更快地稀释添加琥珀酸的比活性。
